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5.1.3.11: cellobiose epimerase

This is an abbreviated version!
For detailed information about cellobiose epimerase, go to the full flat file.

Word Map on EC 5.1.3.11

Reaction

cellobiose
=
4-O-beta-D-glucopyranosyl-D-mannose

Synonyms

B15CE, BfCE, BN699_02313, Caob-CE, CE-NE1, ce13, cellobiose 2-epimerase, CS-HRCE, Csac_0294, CsCE, CvCE, DfCE, Dfer_1975, DsCE, DtCE, Dtur_0652, EcCE, EpiA, epilactose-producing cellobiose 2-epimerase, Epimerase, cellobiose, FbCE, FjCE, Fjoh_4956, HaCE, Haur_0887, mannobiose 2-epimerase, md1, md2, PhCE, Phep_3983, RACE, Rmar_2439, RmCE, SdCE, Sde_0508, SlCE, Slin_0271, Spith_0082, StCE, STHERM_c00950, TERTU_4095, Thsa-CE, TtCE

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.11 cellobiose epimerase

Cloned

Cloned on EC 5.1.3.11 - cellobiose epimerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a random mutant library is prepared via error-prone PCR, identification of mutant R5M/I52V/A12S/K328I/F231L with emhanced enzyme activity and improved performance, recombinant expression of wild-type enzyme and mutants in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli ER2566 cells
-
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli ER2566
expression in Escherichia coli XL-1
expression of His-tagged enzyme in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene B15CE, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose
-
gene bfce, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose
gene BN699_02313, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose
gene ce, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose
gene ce-ne1, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose
gene COB47_2182, sequence comparisons and phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Csac_0294, recombinant expression in Bacillus subtilis strain WB800, subcloning in Escherichia coli strain DH5alpha
gene Csac_0294, recombinant expressison of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene Dfer_1975, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose
gene epiA, recombinant expression in Escherichia coli
-
gene md1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli
gene md2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli
gene Rmar_2439, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose
gene RmCE, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli
gene RmCE, recombinant expression in Escherichia coli
gene Tsac_2329, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
overexpression in Escherichia coli
-
recombinant expression in Escherichia coli
recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant wild type and mutants are expressed in BL21(DE3) Escherichia coli cells
sequence comparisons and phylogenetic tree, recombinant expression
wild-type and mutant enzymes without His-tag are produced in Escherichia coli BL21 (DE3)