5.1.3.11: cellobiose epimerase
This is an abbreviated version!
For detailed information about cellobiose epimerase, go to the full flat file.
Word Map on EC 5.1.3.11
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5.1.3.11
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synthesis
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epimerization
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lactulose
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caldicellulosiruptor
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saccharolyticus
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isomerization
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d-glucose
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ruminococcus
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albus
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prebiotic
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d-mannose
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marinus
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turgidum
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rhodothermus
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nutrition
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dictyoglomus
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mannans
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food industry
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n-acyl-d-glucosamine
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epimerizes
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isomerizes
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cello-oligosaccharides
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spirochaeta
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pharmacology
- 5.1.3.11
- synthesis
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epimerization
- lactulose
- caldicellulosiruptor
- saccharolyticus
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isomerization
- d-glucose
- ruminococcus
- albus
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prebiotic
- d-mannose
- marinus
- turgidum
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rhodothermus
- nutrition
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dictyoglomus
- mannans
- food industry
- n-acyl-d-glucosamine
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epimerizes
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isomerizes
- cello-oligosaccharides
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spirochaeta
- pharmacology
Reaction
Synonyms
B15CE, BfCE, BN699_02313, Caob-CE, CE-NE1, ce13, cellobiose 2-epimerase, CS-HRCE, Csac_0294, CsCE, CvCE, DfCE, Dfer_1975, DsCE, DtCE, Dtur_0652, EcCE, EpiA, epilactose-producing cellobiose 2-epimerase, Epimerase, cellobiose, FbCE, FjCE, Fjoh_4956, HaCE, Haur_0887, mannobiose 2-epimerase, md1, md2, PhCE, Phep_3983, RACE, Rmar_2439, RmCE, SdCE, Sde_0508, SlCE, Slin_0271, Spith_0082, StCE, STHERM_c00950, TERTU_4095, Thsa-CE, TtCE
ECTree
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Application
Application on EC 5.1.3.11 - cellobiose epimerase
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food industry
nutrition
pharmacology
synthesis
the enzyme can be used for production of the probiotic lactulose
food industry
the enzyme is of interest for the dairy industry due to their ability to convert lactose into epilactose and lactulose, rare disaccharides with prebiotic properties
food industry
the enzyme is of interest for the dairy industry due to their ability to convert lactose into epilactose and lactulose, rare disaccharides with prebiotic properties
food industry
the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose
food industry
the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose
food industry
Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3
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the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose
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food industry
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the enzyme is of interest for the dairy industry due to their ability to convert lactose into epilactose and lactulose, rare disaccharides with prebiotic properties
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food industry
Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101
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the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose
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cellobiose 2-epimerase is an attractive enzyme to produce epilactose (4-O-beta-Dgalactosyl-D-mannose) from lactose. Epilactose is a non-digestible oligosaccharide, and enhances proliferation of bifidobacilli and lactobacilli in the human gut
nutrition
cellobiose 2-epimerases can be used for the production of epilactose from milk ultrafiltrate containing lactose
nutrition
cellobiose 2-epimerases can be used for the production of epilactose from milk ultrafiltrate containing lactose
nutrition
the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose
nutrition
the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose
nutrition
Cellulosilyticum lentocellum ATCC 49066 / DSM 5427 / NCIMB 11756 / RHM5
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cellobiose 2-epimerases can be used for the production of epilactose from milk ultrafiltrate containing lactose
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nutrition
Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3
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the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose
-
nutrition
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cellobiose 2-epimerases can be used for the production of epilactose from milk ultrafiltrate containing lactose
-
nutrition
Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101
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the enzyme may introduce an added value for particular dairy products by in situ production of the prebiotic sugar epilactose
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synthesis of lactulose, which is used as a pharmaceutical against various illnesses, such as chronic constipation
pharmacology
synthesis of lactulose, which is used as a pharmaceutical against various illnesses, such as chronic constipation
pharmacology
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synthesis of lactulose, which is used as a pharmaceutical against various illnesses, such as chronic constipation
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cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus is used in the presence of borate to increase the production of lactulose from lactose, method optimization, incubation at pH 7.5 and 80°C for 3 h, with a conversion yield of 88% and a productivity of 205 g/l/h, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
application of the enzyme for production of functional oligosaccharides, overview
synthesis
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application of the enzyme for production of functional oligosaccharides, overview
synthesis
industrial application of Caldicellulosiruptor saccharolyticus cellobiose 2-epimerase (CsCE) for lactulose synthesis is limited by low enzyme activity and formation of epilactose as by-product, the mutant G4-C5, i.e. R5M/I52V/A12S/K328I/F231L, shows improved enzyme activity without compromised thermostability. The yield of lactulose catalyzed by mutant G4-C5 increases to approximately 76% with no obvious epilactose detected, indicating that mutant G4-C5 is more suitable for lactulose production than the wild-type enzyme. The mutant does not produc epilactose as side-product from lactose, in contrast to the wild-type enzyme. Although epilactose is also a bifidus factor, formation of epilactose can complicate lactulose purification
synthesis
industrial application of Caldicellulosiruptor saccharolyticus cellobiose 2-epimerase for lactulose synthesis is limited by low enzyme activity and formation of epilactose as by-product. The yield of lactulose catalyzed by mutant R5M/A12S/I52V/F231L/K328I increases to approximately 76% with no epilactose detected, indicating that the mutant is more suitable for lactulose production than the wild-type enzyme
synthesis
most efficient enzyme for the enzymatic synthesis of lactulose. Ethanol-permeabilised Escherichia coli cells containing the enzyme are used as biocatalysts for lactulose production. The reaction conditions for maximum lactulose production are optimised to be 600 g/l lactose, pH 7.5, 80°C and 12.5 U/ml of whole-cell biocatalyst. After incubated at Na2HPO4-NaH2PO4 buffer for 2 h, approximately 390.59 g/l lactulose is obtained with a conversion yield of 65.1%. The lowest production and conversion yield of epilactose are also found in Na2HPO4-NaH2PO4 buffer with a final concentration of 11.7 g/l and a conversion yield less than 2%. The results represent a promising technology to attain high production and conversion yield of lactulose with a high purity on industrial scale
synthesis
synthesis of lactulose, a non-digestible disaccharide widely used in food and pharmaceutical industries. Thermostability enhancement of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus by site-directed mutagenesis. Compared to the wild-type enzyme the mutant enzyme E161D/N365P shows approximately 4fold increase in the t1/2 value at 80°C and a 1.3fold increase in catalytic efficiency for lactulose production
synthesis
the enzyme can be used for production of the probiotic lactulose
synthesis
the enzyme from Caldicellulosiruptor saccharolyticus is applied for epilactose and lactulose production in milk at lower temperatures
synthesis
the enzyme mutant Y114E can be used for production of lactulose for the nutritional industry. Using Y114E, isomerization of lactose to lactulose is optimnized, resulting in 86.9 g/l of lactulose and 4.6 g/l of epilactose for 2 h from 200 g/l of lactose
synthesis
the recombinant enzyme shows both epimerisation and isomerisation activities against lactose, making it an alternative promising biocatalyst candidate for lactulose production
synthesis
Bacteroides fragilis ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Spirochaeta thermophila ATCC 49972 / DSM 6192 / RI 19.B1
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331
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most efficient enzyme for the enzymatic synthesis of lactulose. Ethanol-permeabilised Escherichia coli cells containing the enzyme are used as biocatalysts for lactulose production. The reaction conditions for maximum lactulose production are optimised to be 600 g/l lactose, pH 7.5, 80°C and 12.5 U/ml of whole-cell biocatalyst. After incubated at Na2HPO4-NaH2PO4 buffer for 2 h, approximately 390.59 g/l lactulose is obtained with a conversion yield of 65.1%. The lowest production and conversion yield of epilactose are also found in Na2HPO4-NaH2PO4 buffer with a final concentration of 11.7 g/l and a conversion yield less than 2%. The results represent a promising technology to attain high production and conversion yield of lactulose with a high purity on industrial scale
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synthesis
Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331
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the enzyme mutant Y114E can be used for production of lactulose for the nutritional industry. Using Y114E, isomerization of lactose to lactulose is optimnized, resulting in 86.9 g/l of lactulose and 4.6 g/l of epilactose for 2 h from 200 g/l of lactose
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synthesis
Herpetosiphon aurantiacus ATCC 23779 / DSM 785
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Spirosoma linguale ATCC 33905 / DSM 74 / LMG 10896
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Saccharophagus degradans 2-40 / ATCC 43961 / DSM 17024
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Teredinibacter turnerae ATCC 39867 / T7901
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Caldicellulosiruptor obsidiansis ATCC BAA-2073 / strain OB47
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the recombinant enzyme shows both epimerisation and isomerisation activities against lactose, making it an alternative promising biocatalyst candidate for lactulose production
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synthesis
Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Dictyoglomus turgidum Z-1310 / DSM 6724
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application of the enzyme for production of functional oligosaccharides, overview
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synthesis
Spirochaeta thermophila ATCC 700085 / DSM 6578 / Z-1203
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application of the enzyme for production of functional oligosaccharides, overview
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