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46 - 147
(2R)-2-naphthylglycolate
0.9 - 2.6
(R)-2-chloromandelate
240
(R)-2-hydroxy-3-butenoic acid
-
-
5.4 - 147
(R)-2-naphthylglycolate
5.4 - 147
(R)-2-naphtylglycolate
-
388 - 2020
(R)-3-chloromandelate
11.03 - 21.17
(R)-mandelamide
0.018 - 1150
(R)-mandelate
2
(R)-trifluorolactate
pH 7.5, 25°C, recombinant wild-type enzyme
0.99 - 3.49
(S)-2-chloromandelate
250
(S)-2-Hydroxy-3-butenoic acid
-
-
2.95 - 101
(S)-2-naphthylglycolate
0.012 - 1124
(S)-Mandelate
2.5
(S)-trifluorolactate
pH 7.5, 25°C, recombinant wild-type enzyme
additional information
additional information
-
46
(2R)-2-naphthylglycolate
pH 7.5, 25°C, wild-type enzyme
70
(2R)-2-naphthylglycolate
pH 7.5, 25°C, wild-type enzyme, in presence of 20 mM MgCl2
137
(2R)-2-naphthylglycolate
pH 7.5, 25°C, mutant V29L
147
(2R)-2-naphthylglycolate
pH 7.5, 25°C, mutant V22I
0.9
(R)-2-chloromandelate
wild-type enzyme, pH 7.5, 25°C
1.23
(R)-2-chloromandelate
mutant Y45F, pH 7.5, 25°C
1.45
(R)-2-chloromandelate
mutant V29I, pH 7.5, 25°C
1.73
(R)-2-chloromandelate
mutant V29I/Y45F, pH 7.5, 25°C
1.82
(R)-2-chloromandelate
mutant V22I, pH 7.5, 25°C
2.4
(R)-2-chloromandelate
mutant V22I/Y45F, pH 7.5, 25°C
2.57
(R)-2-chloromandelate
mutant V22I/V29I/Y54F, pH 7.5, 25°C
2.6
(R)-2-chloromandelate
mutant V22I/V29I, pH 7.5, 25°C
5.4
(R)-2-naphthylglycolate
mutant A25V, pH 7.5, 25°C
7.3
(R)-2-naphthylglycolate
mutant V26F, pH 7.5, 25°C
10.4
(R)-2-naphthylglycolate
mutant V22F, pH 7.5, 25°C
10.5
(R)-2-naphthylglycolate
mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+
14
(R)-2-naphthylglycolate
mutant V26L, pH 7.5, 25°C
14.1
(R)-2-naphthylglycolate
mutant V29F, pH 7.5, 25°C
15.6
(R)-2-naphthylglycolate
mutant V22A, pH 7.5, 25°C
18.3
(R)-2-naphthylglycolate
mutant T24S, pH 7.5, 25°C
23
(R)-2-naphthylglycolate
mutant V26A/V29L, pH 7.5, 25°C
30
(R)-2-naphthylglycolate
mutant V26A, pH 7.5, 25°C
33
(R)-2-naphthylglycolate
25°C, pH 7.5
46
(R)-2-naphthylglycolate
wild-type, pH 7.5, 25°C
53
(R)-2-naphthylglycolate
mutant V29A, pH 7.5, 25°C
70
(R)-2-naphthylglycolate
wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+
137
(R)-2-naphthylglycolate
mutant V29L, pH 7.5, 25°C
147
(R)-2-naphthylglycolate
mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+
5.4
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant A25V
-
7.3
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V26F
-
10.4
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V22F
-
10.5
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V22I/V29L
-
14
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V26L
-
14.1
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V29F
-
15.6
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V22A
-
18.3
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant T24S
-
23
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L
-
30
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V26A
-
46
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, wild-type
-
53
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V29A
-
70
(R)-2-naphtylglycolate
pH 7.5, 20 mM Mg2+, wild-type
-
137
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V29L
-
147
(R)-2-naphtylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V22I
-
388
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V29L
469
(R)-3-chloromandelate
pH 7.5, 25°C, wild-type enzyme
507
(R)-3-chloromandelate
pH 7.5, 25°C, mutant Y54F
510
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V26I/Y54V
559
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V26S
565
(R)-3-chloromandelate
pH 7.5, 25°C, mutant Y54L
637
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V22I
913
(R)-3-chloromandelate
pH 7.5, 25°C, mutant L319F
953
(R)-3-chloromandelate
pH 7.5, 25°C, mutant Y54A
1030
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V26I/Y54I
1160
(R)-3-chloromandelate
pH 7.5, 25°C, mutant Y54V
1220
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V22L
1520
(R)-3-chloromandelate
pH 7.5, 25°C, mutant Y54I
1710
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V26L
2020
(R)-3-chloromandelate
pH 7.5, 25°C, mutant V26I
11.03
(R)-mandelamide
pH 7.5, 25°C, wild-type enzyme
21.17
(R)-mandelamide
pH 7.5, 25°C, mutant V26L
0.018
(R)-mandelate
D270N mutant
0.11
(R)-mandelate
-
mutant E317Q
0.21
(R)-mandelate
mutant K166R
1
(R)-mandelate
mutant enzyme F52W/Y64W
3 - 6
(R)-mandelate
mutant V26L, pH 7.5, 25°C
3 - 6
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V26L
10
(R)-mandelate
mutant enzyme Y54W
13
(R)-mandelate
mutant A25V, pH 7.5, 25°C
13
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant A25V
16.9
(R)-mandelate
-
25°C, pH 7.5, mutant enzyme N197A
33
(R)-mandelate
mutant V26F, pH 7.5, 25°C
33
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V26F
53
(R)-mandelate
mutant V29F, pH 7.5, 25°C
53
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V29F
82
(R)-mandelate
mutant V22A, pH 7.5, 25°C
82
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V22A
98
(R)-mandelate
mutant V22F, pH 7.5, 25°C
98
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V22F
102
(R)-mandelate
mutant T24S, pH 7.5, 25°C
102
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant T24S
106
(R)-mandelate
mutant V26A/V29L, pH 7.5, 25°C
106
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L
159
(R)-mandelate
mutant enzyme F52W
240
(R)-mandelate
mutant V29A, pH 7.5, 25°C
240
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V29A
300
(R)-mandelate
mutant V29L, pH 7.5, 25°C
300
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V29L
304
(R)-mandelate
mutant V26A, pH 7.5, 25°C
304
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V26A
480
(R)-mandelate
-
wild type enzyme
500
(R)-mandelate
-
wild type enzyme
514
(R)-mandelate
-
25°C, pH 7.5, wild-type enzyme
526
(R)-mandelate
wild-type enzyme
552
(R)-mandelate
wild-type, pH 7.5, 25°C
552
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, wild-type
620
(R)-mandelate
pH 7.5, 20 mM Mg2+, wild-type
620
(R)-mandelate
wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+
792
(R)-mandelate
pH 7.5, 25°C, recombinant wild-type enzyme
1010
(R)-mandelate
mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+
1010
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V22I/V29L
1150
(R)-mandelate
mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+
1150
(R)-mandelate
pH 7.5, 3.3 mM Mg2+, mutant V22I
0.99
(S)-2-chloromandelate
wild-type enzyme, pH 7.5, 25°C
1.33
(S)-2-chloromandelate
mutant Y45F, pH 7.5, 25°C
1.83
(S)-2-chloromandelate
mutant V29I, pH 7.5, 25°C
1.96
(S)-2-chloromandelate
mutant V29I/Y45F, pH 7.5, 25°C
2.35
(S)-2-chloromandelate
mutant V22I/V29I, pH 7.5, 25°C
2.47
(S)-2-chloromandelate
mutant V22I, pH 7.5, 25°C
2.51
(S)-2-chloromandelate
mutant V22I/Y45F, pH 7.5, 25°C
3.49
(S)-2-chloromandelate
mutant V22I/V29I/Y54F, pH 7.5, 25°C
2.95
(S)-2-naphthylglycolate
mutant V22F, pH 7.5, 25°C
2.95
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V22F
5.4
(S)-2-naphthylglycolate
mutant V26F, pH 7.5, 25°C
5.4
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V26F
5.8
(S)-2-naphthylglycolate
mutant A25V, pH 7.5, 25°C
5.8
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant A25V
7.1
(S)-2-naphthylglycolate
mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+
7.1
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L
8.2
(S)-2-naphthylglycolate
mutant V26L, pH 7.5, 25°C
8.2
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V26L
8.9
(S)-2-naphthylglycolate
mutant T24S, pH 7.5, 25°C
8.9
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant T24S
9.7
(S)-2-naphthylglycolate
mutant V29F, pH 7.5, 25°C
9.7
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V29F
9.8
(S)-2-naphthylglycolate
mutant V26A/V29L, pH 7.5, 25°C
9.8
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L
11.1
(S)-2-naphthylglycolate
mutant V22A, pH 7.5, 25°C
11.1
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V22A
14
(S)-2-naphthylglycolate
mutant V26A, pH 7.5, 25°C
14
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V26A
25
(S)-2-naphthylglycolate
25°C, pH 7.5
30
(S)-2-naphthylglycolate
mutant V29A, pH 7.5, 25°C
30
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V29A
32
(S)-2-naphthylglycolate
wild-type, pH 7.5, 25°C
32
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, wild-type
38.4
(S)-2-naphthylglycolate
pH 7.5, 20 mM Mg2+, wild-type
38.4
(S)-2-naphthylglycolate
wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+
65
(S)-2-naphthylglycolate
mutant V29L, pH 7.5, 25°C
65
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V29L
101
(S)-2-naphthylglycolate
mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+
101
(S)-2-naphthylglycolate
pH 7.5, 3.3 mM Mg2+, mutant V22I
0.012
(S)-Mandelate
-
mutant E317Q
0.037
(S)-Mandelate
D270N mutant
0.46
(S)-Mandelate
-
mutant K166R
2 - 8
(S)-Mandelate
mutant enzyme Y54W
2.5
(S)-Mandelate
-
25°C, pH 7.5, mutant enzyme N197A
12
(S)-Mandelate
mutant enzyme F52W/Y64W
22
(S)-Mandelate
mutant A25V, pH 7.5, 25°C
22
(S)-Mandelate
mutant V26F, pH 7.5, 25°C
22
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant A25V
22
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant V26F
23
(S)-Mandelate
mutant V26L, pH 7.5, 25°C
23
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant V26L
53
(S)-Mandelate
mutant V29F, pH 7.5, 25°C
53
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant V29F
77
(S)-Mandelate
mutant V22A, pH 7.5, 25°C
77
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+ mutant V22A
89
(S)-Mandelate
mutant V26A/V29L, pH 7.5, 25°C
89
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L
98
(S)-Mandelate
mutant V22F, pH 7.5, 25°C
98
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant V22F
120
(S)-Mandelate
mutant T24S, pH 7.5, 25°C
120
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant T24S
176
(S)-Mandelate
mutant V29A, pH 7.5, 25°C
176
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant V29A
180
(S)-Mandelate
mutant V29L, pH 7.5, 25°C
180
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant V29L
190
(S)-Mandelate
recombinant mandelate racemase, putative misfolded form with N-terminal hexahistidine tag, Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2
248
(S)-Mandelate
mutant enzyme F52W
350
(S)-Mandelate
-
wild type enzyme
440
(S)-Mandelate
-
wild type enzyme
447
(S)-Mandelate
-
25°C, pH 7.5, wild-type enzyme
467
(S)-Mandelate
wild-type enzyme
470
(S)-Mandelate
wild-type, pH 7.5, 25°C
470
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, wild-type
472
(S)-Mandelate
recombinant mandelate racemase fusion protein bearing an N-terminal StrepII-tag and a C-terminal decahistidine tag, Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2
492
(S)-Mandelate
pH 7.5, 20 mM Mg2+, wild-type
492
(S)-Mandelate
wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+
550
(S)-Mandelate
mutant V26A, pH 7.5, 25°C
550
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant V26A
637
(S)-Mandelate
pH 7.5, 25°C, recombinant wild-type enzyme
710
(S)-Mandelate
mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+
710
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L
740
(S)-Mandelate
mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+
740
(S)-Mandelate
pH 7.5, 3.3 mM Mg2+, mutant V22I
940
(S)-Mandelate
recombinant mandelate racemase represents correctly folded enzyme, with N-terminal hexahistidine tag, in Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2
1124
(S)-Mandelate
recombinant enzyme replacement of the N-terminal hexahistidine tag by a StrepII-tag appears to ameliorate the folding problem yielding a single enzyme species, Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2
additional information
additional information
-
dependence of turnover number on pH
-
additional information
additional information
dependence of turnover number on pH
-
additional information
additional information
-
dependence of turnover number on pH
-
additional information
additional information
-
the turnover number of the mutant enzyme is unaffected by varying solvent viscosity
-
5.1.2.2 mandelate racemase
results (
results (
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