5.1.2.2: mandelate racemase
This is an abbreviated version!
For detailed information about mandelate racemase, go to the full flat file.
Word Map on EC 5.1.2.2
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5.1.2.2
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s-mandelate
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kenyon
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alpha-proton
-
muconate
-
gerlt
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1,1-proton
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benzohydroxamate
-
petsko
-
kozarich
-
mitra
-
galactarate
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benzoylformate
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d-glucarate
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ransom
-
synthesis
-
analysis
-
biotechnology
- 5.1.2.2
- s-mandelate
-
kenyon
-
alpha-proton
- muconate
-
gerlt
-
1,1-proton
- benzohydroxamate
-
petsko
-
kozarich
- mitra
- galactarate
- benzoylformate
- d-glucarate
-
ransom
- synthesis
- analysis
- biotechnology
Reaction
Synonyms
mandelate racemase, mandelic acid racemase, mdlA, MR, racemase, mandelate
ECTree
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KCat KM Value
KCat KM Value on EC 5.1.2.2 - mandelate racemase
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1.6
(R)-trifluorolactate
pH 7.5, 25°C, recombinant wild-type enzyme
1.4
(S)-trifluorolactate
pH 7.5, 25°C, recombinant wild-type enzyme
20.1
pH 7.5, 25°C, wild-type enzyme, in presence of 20 mM MgCl2
1100
(S)-Mandelate
recombinant mandelate racemase, replacement of the N-terminal hexahistidine tag by a StrepII-tag appears to ameliorate the folding problem yielding a single enzyme species, Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2
1200
(S)-Mandelate
recombinant mandelate racemase, represents correctly folded enzyme with N-terminal hexahistidine tag, in Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2
7300
(S)-Mandelate
recombinant mandelate racemase, putative misfolded form with N-terminal hexahistidine tag, Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2
8000
(S)-Mandelate
recombinant mandelate racemase, fusion protein bearing an N-terminal StrepII-tag and a C-terminal decahistidine tag, Na-HEPES buffer (0.1 M, pH 7.5), 3.3 mM MgCl2