5.1.2.2: mandelate racemase

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For detailed information about mandelate racemase, go to the full flat file.

Word Map on EC 5.1.2.2

5.1.2.2

s-mandelate

kenyon

alpha-proton

muconate

gerlt

1,1-proton

benzohydroxamate

petsko

kozarich

mitra

galactarate

benzoylformate

d-glucarate

ransom

synthesis

analysis

biotechnology

Reaction

Synonyms

mandelate racemase, mandelic acid racemase, mdlA, MR, racemase, mandelate

ECTree

5 Isomerases

5.1 Racemases and epimerases

5.1.2 Acting on hydroxy acids and derivatives

5.1.2.2 mandelate racemase

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Results	in table
4	Activating Compound
467	AA Sequence
7	Application
1	CAS Registry Number
9	Cloned(Commentary)
10	Crystallization (Commentary)
10	General Information
1	General Stability
81	Inhibitors
45	kcat/KM [mM/s]
57	Ki Value [mM]
223	KM Value [mM]
32	Metals/Ions
12	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
64	Organism
6	Pathway
53	PDB ID
10	pH Optimum
78	Protein Variants
11	Purification (Commentary)
15	Reaction
2	Reaction Type
60	Reference
21	Specific Activity [micromol/min/mg]
118	Substrates and Products (Substrate)
5	Subunits
12	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
1	Temperature Stability [°C]
198	Turnover Number [1/s]

General Stability

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General Stability on EC 5.1.2.2 - mandelate racemase

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recombinant enzyme exhibits only a slight loss of activity when the enzyme is dialyzed without 200 mM NaCl at 4°C for 8 h, a loss of 20% of its activity when the enzyme is stored for 1 h on ice in the absence of BSA (but stable in the presence of 0.1% BSA), and a 30% reduction in Vmax after storage for 20 days at -70°C in the presence of 200 mM NaCl

Pseudomonas putida

P11444

706599