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5.1.2.2: mandelate racemase

This is an abbreviated version!
For detailed information about mandelate racemase, go to the full flat file.

Word Map on EC 5.1.2.2

Reaction

(S)-Mandelate
=
(R)-mandelate

Synonyms

mandelate racemase, mandelic acid racemase, mdlA, MR, racemase, mandelate

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.2 Acting on hydroxy acids and derivatives
                5.1.2.2 mandelate racemase

Cloned

Cloned on EC 5.1.2.2 - mandelate racemase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of fusions proteins in Escherichia coli
expressed ind Escherichia coli
-
expression in Escherichia coli
-
gene mdlA, coexpression of mandelate racemase and mandelate dehydrogenase in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain TG1
gene mdlA, genetic library construction, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
overexpression in Escherichia coli strain BL21(DE3)
overexpression of Strep-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Recombinant MR from is overproduced from Escherichia coli strain BL21(DE3) cells transformed with a pET-15b plasmid containing the MR open reading frame. This construct encodes the MR gene product with an N-terminal hexahistidine tag. Mutants are produced by site-directed mutagenesis