5.1.1.5: lysine racemase

This is an abbreviated version!
For detailed information about lysine racemase, go to the full flat file.

Reaction

Synonyms

lyr, lysine racemase, Racemase, lysine

ECTree

     5 Isomerases

         5.1 Racemases and epimerases

             5.1.1 Acting on amino acids and derivatives

                5.1.1.5 lysine racemase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
11	AA Sequence
4	Application
1	CAS Registry Number
7	Cloned(Commentary)
4	Cofactor
1	Crystallization (Commentary)
1	Expression
4	General Information
4	Inhibitors
16	kcat/KM [mM/s]
28	KM Value [mM]
5	Metals/Ions
2	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
14	Organism
6	Pathway
1	PDB ID
6	pH Optimum
1	pH Range
1	pH Stability
25	Protein Variants
3	Purification (Commentary)
1	Reaction
3	Reaction Type
12	Reference
13	Specific Activity [micromol/min/mg]
26	Substrates and Products (Substrate)
3	Subunits
11	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
28	Turnover Number [1/s]

Specific Activity

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Specific Activity on EC 5.1.1.5 - lysine racemase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Specific Activity Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

1.68

uncultured bacterium

C7ACH5

substrate 10 mM D-lysine, in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C

701800

1043

Proteus mirabilis

M4GGR9

purified recombinant His-tagged mutant S394T, substrate L-arginine, pH 8.0, 50°C

728711

1185

Proteus mirabilis

M4GGR9

purified recombinant His-tagged mutant S394Y, substrate L-arginine, pH 8.0, 50°C

728711

1200

Proteus mirabilis

M4GGR9

purified recombinant His-tagged mutant S394N, substrate L-arginine, pH 8.0, 50°C

728711

1633

Proteus mirabilis

M4GGR9

purified recombinant His-tagged mutant S394N, substrate L-lysine, pH 8.0, 50°C

728711

2356

Proteus mirabilis

M4GGR9

purified recombinant His-tagged mutant S394C, substrate L-lysine, pH 8.0, 50°C

728711

2828

Proteus mirabilis

M4GGR9

purified recombinant His-tagged enzyme, substrate L-lysine, pH 8.0, 50°C

728711

3.61

uncultured bacterium

C7ACH5

substrate 10 mM L-lysine, in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C

701800

3127

Proteus mirabilis

M4GGR9

purified recombinant His-tagged mutant S394T, substrate L-lysine, pH 8.0, 50°C

728711

568

Proteus mirabilis

M4GGR9

purified recombinant His-tagged enzyme, substrate L-arginine, pH 8.0, 50°C

728711

887

Proteus mirabilis

M4GGR9

purified recombinant His-tagged mutant S394Y, substrate L-lysine, pH 8.0, 50°C

728711

980

Proteus mirabilis

M4GGR9

purified recombinant His-tagged mutant S394C, substrate L-arginine, pH 8.0, 50°C

728711

additional information

uncultured bacterium

C7ACH5

enzyme is incubated in 1 ml of reaction mixture containing 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride and 10 mM L- or D-lysine at 30°C, Lyr activities in 4 week old wild type and transgenic tobacco plants are determined, the specific activity of Lyr in the transgenic T2 plants selected on L-lysine ranges from 0.77 to 1.06 mU/mg protein, although transgenic plants exhibit considerable variation in enzyme activities, no phenotypic dissimilarities associated with L-lysine selection are found, suggesting that the Lyr gene as a selectable marker could be effective over a range of expression levels

706257