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5.1.1.18: serine racemase

This is an abbreviated version!
For detailed information about serine racemase, go to the full flat file.

Word Map on EC 5.1.1.18

Reaction

L-serine
=
D-serine

Synonyms

hSR, More, RiSR, RLO149_c015450, Ser racemase, SerR, SRace, SRR, T01H8.2, Zm-SR, ZmSR

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.18 serine racemase

Purification

Purification on EC 5.1.1.18 - serine racemase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
both isoforms A and B
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expression in Escherichia coli with N-terminal His-tag, purification protocol from inclusion bodies
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native enzyme by anion exchange chromatography, recombinant enzyme from Escherichia coli by treatment with L-arginine and anion exchange chromatography
Ni-NTA agarose column chromatography
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purification of isozyme I, partial purification of isozyme II
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recombinant C-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain MC1061 by nickel affinity chromatography and gel filtration
recombinant enzyme
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recombinant enzyme expressed in insect cells
recombinant enzyme from Escherichia coli strain MC1061 by hydrophobic interaction and anion exchange chromatography, followed by ATP affinity chromatography and dialysis
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recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis, to near homogeneity
recombinant His-tagged enzyme from Escherichia coli nickel affinity chromatography and dialysis, to near homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by metal affinity chromatography
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recombinant His-tagged enzyme mutant from Escherichia coli strain Rosetta 2 (DE3) by affinity chromatography and gel filtration to over 98% purity
recombinant His-tagged SerR 16.9fold from Escherichia coli strain BL21 (DE3) to homogeneity ny nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta II (DE3) by nickel affinity chromatography
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recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21 CodonPlus (DE3)-RIL by nickel affinity chromatography
recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 CodonPlus (DE3)-RIL
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) using Talon resin chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and gel filtration
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