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5.1.1.18: serine racemase

This is an abbreviated version!
For detailed information about serine racemase, go to the full flat file.

Word Map on EC 5.1.1.18

Reaction

L-serine
=
D-serine

Synonyms

hSR, More, RiSR, RLO149_c015450, Ser racemase, SerR, SRace, SRR, T01H8.2, Zm-SR, ZmSR

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.18 serine racemase

Cloned

Cloned on EC 5.1.1.18 - serine racemase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from genomic DNA, DNA and amino acid sequence determination
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strains JM109 and BL21(DE3)
enzyme expression in Escherichia coli strain MC1061
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enzyme expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL
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expressed in Escherichia coli BL21(DE3) cells
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expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain MC1061
expression of GST-tagged fusion protein containing amino acid residue 150-190 of the mouse enzyme, expression of His-tagged enzyme in Cavia porcellus and Oryctolagus cuniculus, quantitative real-time PCR enzyme expression analysis
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expression of His-tagged enzyme mutant in Escherichia coli strain Rosetta 2 (DE3)
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta II (DE3)
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expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of the highly active His-tagged serine racemase from brain in moderate halophilic bacteria, the recombinant enzyme shows 2.6fold higher elimination than racemization activity
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expression of untagged and of N-terminally His6-tagged and C-terminally FLAG-tagged enzyme in COS-7 cells, co-expression with glutamate receptor interacting protein or FLAG-tagged glutamate receptor interacting protein domain PDZ6
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expression oof wild-type and mutant enzyme in Escherichia coli strain Rosetta 2 (DE3)
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gene alr2, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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gene serR, encoding the enzyme, is located on chromosome 4 of the genomic DNA, phylogenetic analysis, cloning and expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene Srr, DNA and amino acid sequence determination and analysis, the single guide (sg) RNA target sequence in the exon 4 of Srr are annealed and subcloned into the BsbI site of pX362 to yield the pXSrre4T1, the circular pXSrre4T1 plasmid is transfected into PC-12 cells, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)
gene srr, DNA and amino acid sequence determination and anaylsis, expression in Escherichia coli
gene Srr, recombinant expression of wild-type and mutant enzymes in Rattus norvegicus PC-12 cells, recombinant expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3). SRR-overexpressing PC-12 cells produce endogenous D-Asp and D-Ser
gene SRR, sequence comparisons and phylogenetic tree
gene T01H8.2, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli. Other than the T01H8.2 gene, there is no known gene within the Caenorhabditis elegans genome that is orthologous to a Ser racemase gene
HEK293 cells transfected with wild-type enzyme and metabotropic glutamate receptor display a 4fold increase in enzyme activity upon treatment with the metabotropic glutamate receptor agonist dihydroxyphenylglycine
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optimization for expression in Escherichia coli
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overexpression in ATDC5 cell and in COS7 cell
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overexpression of HA-tagged enzyme in SH-SY5Y neuroblastoma cells and in HEK293 cells, coexpression with palmitic acid leads to acylation of the enzyme
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quantitative expression analysis
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recombinant expression of His-tagged wild-type and mutant enzymes in HEK-293T cells
recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21 CodonPlus (DE3)-RIL
recombinant expression of N-terminally His-tagged enzyme in Escherichia coli
sequence comparisons and phylogenetic tree
sequence comparisons, cloning from leaf DNA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
sequence comparisons, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 CodonPlus (DE3)-RIL
total RNA is isolated from rMC-1 cells reverse-transcribed into cDNA and subjected to PCR using rat-specific SR primers, expression analysis of the enzyme in retinal Mueller cells