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5.1.1.15: 2-aminohexano-6-lactam racemase

This is an abbreviated version!
For detailed information about 2-aminohexano-6-lactam racemase, go to the full flat file.

Word Map on EC 5.1.1.15

Reaction

(S)-2-aminohexano-6-lactam
=
(R)-2-aminohexano-6-lactam

Synonyms

ACL racemase, ACLR, alpha-Amino-delta-valerolactam racemase, alpha-Amino-epsilon-caprolactam racemase, Aminocaprolactam racemase, moe, More, Racemase, alpha-amino-epsilon-caprolactam, Racemase, alpha-amino-epsilon-caprolactam (Achromobacter obae reduced), SMc02413

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.15 2-aminohexano-6-lactam racemase

Purification

Purification on EC 5.1.1.15 - 2-aminohexano-6-lactam racemase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ACL racemase is purified from Escherichia coli JM109/pACL60
-
native enzyme by several steps of ion exchange and hydrophobic interaction chromatography, dialysis, ultrafiltration, and gel filtration, recombinant His-tagged enzyme 31.9fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
-
recombinant
-
recombinant His-tagged enzyme 129fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
-
recombinant His-tagged enzyme 27.9fold from Escherichia coli strain BL21(DE3) by heat treatment, nickel affinity chromatography, and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis
using Ni Sepharose highperformance (Amersham) and anion-exchange chromatography (HiTrapQFF)