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5.1.1.13: aspartate racemase

This is an abbreviated version!
For detailed information about aspartate racemase, go to the full flat file.

Word Map on EC 5.1.1.13

Reaction

L-aspartate
=
D-Aspartate

Synonyms

Asp racemase, Aspartate racemase, AspR, D-Asp racemase, D-Aspartate racemase, GOT1L1, LsAspR, OCC_11152, P. AspR, PhAspR, PLP-independent AspR, PTO0149, PtoAspR, pyridoxal 5'-phosphate independent aspartate racemase, pyridoxal 5'-phosphate-independent aspartate racemase, Racemase, aspartate, SbAspR, Tl-AspR

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.13 aspartate racemase

Purification

Purification on EC 5.1.1.13 - aspartate racemase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
aspartate racemase is purified from the Scapharca broughtonii foot muscle by a procedure that includes sodium sulfate fractionation and blue sepharose column chromatography. The purified enzyme (225 mg) is digested at 25°C for 12 h with 80 mg of lysyl endopeptidase in a reaction mixture comprising 50 mM Tris-HCl, pH 8.0, containing 2 M guanidine hydrochloride, and the resulting peptides is resolved by reverse phase high performance liquid chromatography on a capcell Pac C-8 column (0.2 mm x 15 cm) using an acetonitrile gradient in 0.06% trifluoroacetic acid
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His-tagged aspartate racemase is purified on Talon metal affinity resin
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recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration
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recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
recombinant C-terminally His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction chromatography, dialysis anion change chromatography, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography
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recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21 and HEK-293T cells by nickel affinity chromatography and desalting gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) by heat treatment of cell-free extract at 70°C for 30 min, centrifugation, dialysis, and anion exchange chromatography, dialysis, hydrophobic interaction chromatography, and another different anion exchange chromatography, followed by dialysis