Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

5.1.1.11: phenylalanine racemase (ATP-hydrolysing)

This is an abbreviated version!
For detailed information about phenylalanine racemase (ATP-hydrolysing), go to the full flat file.

Word Map on EC 5.1.1.11

Reaction

ATP
+
L-phenylalanine
+
H2O
=
AMP
+
diphosphate
+
D-phenylalanine

Synonyms

Gramicidin S synthetase I, GrsA, GS I, PheATE, Phenylalanine racemase, Phenylalanine racemase (adenosine triphosphate-hydrolyzing), Phenylalanine racemase (ATP-hydrolyzing), Racemase, phenylalanine (adenosine triphosphate-hydrolyzing)

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.11 phenylalanine racemase (ATP-hydrolysing)

Engineering

Engineering on EC 5.1.1.11 - phenylalanine racemase (ATP-hydrolysing)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C331A
minor changes in activity or specificity
C331L
activity of GrsA is decreased to 26%
C376S
no changes in activity
C473A
no changes in activity
C60F
no changes in activity
C60F/C331A/C376S/C473A
named A-PCP(delta-4Cys), Km for L-Phe is increased by 2fold, while the kcat is reduced by 1.5fold
D767S
-
mutant enzyme is impaired in approach to D-Phe/L-Phe-S-4'phosphopantetheine equilibrium only for D-Phe to L-Phe direction
E892A
H753A
-
only slightly active with L-Phe, unimpaired reaction with D-Phe
R896A
-
mutant enzyme is dramatically impaired in approach to D-Phe/L-Phe-S-4'phosphopantetheine equilibrium from either D-Phe or L-Phe