5.1.1.10: amino-acid racemase

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For detailed information about amino-acid racemase, go to the full flat file.

Word Map on EC 5.1.1.10

5.1.1.10

racemization

pyridoxal

synthesis

plp-dependent

alpha-hydrogen

plp-binding

buchneri

5'-phosphate-dependent

2-epimerase

biotechnology

diagnostics

analysis

Reaction

Synonyms

AAR, ACL racemase, amino acid racemase, amino-acid racemase, ArgR, BAR, broad specificity amino acid racemase, broad substrate specificity amino acid racemase, broad-specificity amino acid racemase, broad-spectrum amino acid racemase, Bsar, D-amino acid racemase 1, DAR1, GkNSAAR, L-Amino acid racemase, LACBS_00576, MalY, More, N-succinylamino acid racemase, NSAR, patB, PH0138, PLP-independent amino acid racemase, Racemase, amino acid, RacX, YgeA

ECTree

5 Isomerases

5.1 Racemases and epimerases

5.1.1 Acting on amino acids and derivatives

5.1.1.10 amino-acid racemase

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Results	in table
2	Activating Compound
839	AA Sequence
24	Application
1	CAS Registry Number
28	Cloned(Commentary)
27	Cofactor
3	Crystallization (Commentary)
2	Expression
11	General Information
1	General Stability
26	Inhibitors
52	kcat/KM [mM/s]
152	KM Value [mM]
2	Localization
12	Metals/Ions
37	Molecular Weight [Da]
37	Natural Substrates/ Products (Substrates)
67	Organism
19	Pathway
26	PDB ID
30	pH Optimum
5	pH Range
3	pH Stability
1	Posttranslational Modification
22	Protein Variants
31	Purification (Commentary)
7	Reaction
1	Reaction Type
62	Reference
11	Source Tissue
76	Specific Activity [micromol/min/mg]
1	Storage Stability
437	Substrates and Products (Substrate)
32	Subunits
96	Synonyms
1	Systematic Name
25	Temperature Optimum [°C]
5	Temperature Range [°C]
7	Temperature Stability [°C]
41	Turnover Number [1/s]

Purification

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Purification on EC 5.1.1.10 - amino-acid racemase

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3 entries

both isoforms A and B

Mus musculus

662950

expression in Escherichia coli with N-terminal His-tag, purification protocol from inclusion bodies

Homo sapiens

663323

His-tag is removed by thrombin cleavage

Geobacillus kaustophilus

702711

His6-tagged BAR is applied onto a His-Trap HP 1-ml column

Pseudomonas putida

671544

Ni-NTA column chromatography and Sepharcryl 200 gel filtration

Aplysia californica

715591

protein is purified by applying to a diethylaminoethyl sepharose fast flow column and a phenyl sepharose 6 fast flow column

Geobacillus kaustophilus

672148

purified in a one-step procedure by immobilized cobalt affinity chromatography

Geobacillus kaustophilus

706572

recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography

2 entries

recombinant enzyme expressed in insect cells

Mus musculus

661725

recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis

9 entries

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, to homogeneity

Latilactobacillus sakei

A0A223K4L6

747857

recombinant soluble enzyme BAR from Escherichia coli strain BL21(DE3) by heat treatment at 90°C for 20 min, ammonium sulfate fractionation, and hydrophobic interaction chromatography, dialysis, anion echange chromatography, and again dialysis

Pyrococcus horikoshii

O57878

745414

two enzyme lyophilisates of different purity are obtained from which the crude is sufficient for the racemization of methionine and the pure is used for asparagine

Pseudomonas putida

705194

using Ni-NTA chromatography

Pseudomonas taetrolens

I0J1I6

701839