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5.1.1.10: amino-acid racemase

This is an abbreviated version!
For detailed information about amino-acid racemase, go to the full flat file.

Word Map on EC 5.1.1.10

Reaction

an L-amino acid
=
a D-amino acid

Synonyms

AAR, ACL racemase, amino acid racemase, amino-acid racemase, ArgR, BAR, broad specificity amino acid racemase, broad substrate specificity amino acid racemase, broad-specificity amino acid racemase, broad-spectrum amino acid racemase, Bsar, D-amino acid racemase 1, DAR1, GkNSAAR, L-Amino acid racemase, LACBS_00576, MalY, More, N-succinylamino acid racemase, NSAR, patB, PH0138, PLP-independent amino acid racemase, Racemase, amino acid, RacX, YgeA

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.10 amino-acid racemase

Engineering

Engineering on EC 5.1.1.10 - amino-acid racemase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K233A
site-directed mutagenesis
Y123A
site-directed mutagenesis
K233A
-
site-directed mutagenesis
-
Y123A
-
site-directed mutagenesis
-
H152S
-
ratio of elimination reaction to racemization is 1.4 compared to 3.7 in wild-type
N154F
-
ratio of elimination reaction to racemization is 0.33 compared to 3.7 in wild-type
P153S
-
ratio of elimination reaction to racemization is 0.24 compared to 3.7 in wild-type
Q155D
-
ratio of elimination reaction to racemization is 0.25 compared to 3.7 in wild-type
I384M
I83L/D361V/Y396C
-
specific activity: 0.0303 (substrate Trp), 0.0257 (substrate Phe), 0.473 (substrate Lys), 0.0121 (substrate Ala)
L126H/Y396C
-
specific activity: 0.0524 (substrate Trp), 0.0436 (substrate Phe), 0.477 (substrate Lys), 0.0144 (substrate Ala)
R174A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
R174K
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme. Mutant Bsar-R174K shows no deleterious effects on the transgenic plants and broad range amino acid racemase activity, might serve as a good selection marker
Y293A/Y301S/Y396C
-
specific activity: 0.0267 (substrate Trp), 0.0249 (substrate Phe), 0.458 (substrate Lys), 0.0141 (substrate Ala)
Y396C
-
specific activity: 0.0487 (substrate Trp), 0.00706 (substrate Phe), 0.03215 (substrate Lys), 0.825 (substrate Ala)
R174A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
-
R174K
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme. Mutant Bsar-R174K shows no deleterious effects on the transgenic plants and broad range amino acid racemase activity, might serve as a good selection marker
-
additional information