5.1.1.1: alanine racemase
This is an abbreviated version!
For detailed information about alanine racemase, go to the full flat file.
Word Map on EC 5.1.1.1
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5.1.1.1
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pyridoxal
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5'-phosphate
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peptidoglycan
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racemization
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d-cycloserine
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stearothermophilus
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plp-dependent
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d-amino
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aldimine
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d-alanyl-d-alanine
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exosporium
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pyridoxal-5'-phosphate-dependent
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drug development
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5'-phosphate-dependent
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d-alanine:d-alanine
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medicine
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biotechnology
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pharmacology
- 5.1.1.1
- pyridoxal
- 5'-phosphate
- peptidoglycan
-
racemization
- d-cycloserine
- stearothermophilus
-
plp-dependent
-
d-amino
-
aldimine
- d-alanyl-d-alanine
- exosporium
-
pyridoxal-5'-phosphate-dependent
- drug development
-
5'-phosphate-dependent
-
d-alanine:d-alanine
- medicine
- biotechnology
- pharmacology
Reaction
Synonyms
1SFT, AAR, alanine racemase, AlaR, ALR, alr-2, ALR1, ALR2, Alr2 racemase, AlrA, AlrAba, AlrBax, AlrMtb, alrTt, ARL, BA0252, BAS0238, CBL/ALR, CdAlr, cystathionine beta-lyase, D-alanine racemase, DadB, DadX, dadXOF4, dal1, EcAlr, EcCBL, EfAlaR, L-Alanine racemase, L-Alanine:D-alanine racemase, MBalr1, MBAlr2, MetC, More, MurI, OEOE_1641, PDB, Racemase, alanine, tAlaRac, TmCBL, wMelCBL
ECTree
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Temperature Stability
Temperature Stability on EC 5.1.1.1 - alanine racemase
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0 - 50
purified recombinant enzyme, stable up to, over 90% activity remaining
25 - 80
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95% activity at 25°C, 80% activity at 40°C, less than 20% activity at 50°C, less than 10% activity at 60 and 70°C, inactive at 80°C
25 - 85
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between 60 and 70°C 50% of the enzyme activity is lost, at 85°C 50% of the protein molecules are denatured, the residual activity is 4%
30
35
pH 8.3, 1 h, the purified is extremely labile over 35°C
40
50
60
70
80
purified recombinant wild-type enzyme, sharp decline during incubation at 80°C, half-life is 1.5 h
95
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5 min, resistant to incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide
30
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5 min, about 50% loss of activity after incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide
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the decrease in the enzyme activity at incubation temperatures over 40°C is consistent with the decrease in the amount of bound pyridoxal 5'-phosphate, no effect on stability at lower temperatures, 0-30°C
40
1 h, quick loss of activity in absence of pyridoxal 5'-phosphate
purified recombinant wild-type enzyme, 12 h, completely stable, the first several hours of incubation at 50-70°C help in thermoactivation of the enzyme
50
pH 8.3, 1 h, stabilized up to 50°C in presence of excess amounts of pyridoxal 5'-phosphate, 0.4 mM, complete inactivation in absence of pyridoxal 5'-phosphate
50
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate
50
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate
50
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate
50
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate
60
purified recombinant wild-type enzyme, 3 h, completely stable, the first several hours of incubation at 50-70°C help in thermoactivation of the enzyme
70
purified recombinant wild-type enzyme, 3 h, completely stable, half-life is 12 h, the first several hours of incubation at 50-70°C help in thermoactivation of the enzyme
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pH 8.0, 50 mM Tris-HCl, 0.5 M sodium phosphate, maintains more than 90% of its activity after 2 h