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5.1.1.1: alanine racemase

This is an abbreviated version!
For detailed information about alanine racemase, go to the full flat file.

Word Map on EC 5.1.1.1

Reaction

L-alanine
=
D-alanine

Synonyms

1SFT, AAR, alanine racemase, AlaR, ALR, alr-2, ALR1, ALR2, Alr2 racemase, AlrA, AlrAba, AlrBax, AlrMtb, alrTt, ARL, BA0252, BAS0238, CBL/ALR, CdAlr, cystathionine beta-lyase, D-alanine racemase, DadB, DadX, dadXOF4, dal1, EcAlr, EcCBL, EfAlaR, L-Alanine racemase, L-Alanine:D-alanine racemase, MBalr1, MBAlr2, MetC, More, MurI, OEOE_1641, PDB, Racemase, alanine, tAlaRac, TmCBL, wMelCBL

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.1 alanine racemase

Purification

Purification on EC 5.1.1.1 - alanine racemase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
alanine racemase is purified from the muscle of C. grata by using Toyopearl DEAE, Toyopearl Butyl, Hydroxyapatite and Sephacryl S200 column chromatographies.
-
ammonium sulfate fractionation
-
ammonium sulfate precipitation, anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration
by Ether-Toyopearl, Phenyl-Toyopearl and DEAE-Toyopearl column chromatographic steps
-
enzyme overproduced in Escherichia coli W3110 lacIq
-
Ni2+-NTA column chromatography and Sephacryl S-200 gel filtration
-
Ni2+-NTA resin (His-tagged overexpressed protein), Hitrap Q-column, Superdex S prep grad column
-
partial
partial, from stationary growth phase
-
purified to electrophoretic homogeneity
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant C-terminally His6-tagged selenomethionine-derivatized enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant codon-optimized wild-type and mutant enzymes from Escherichia coli strain Rosetta 2 (pLysS) DE3 by anion exchange chromatography, gel filtration, and dialysis
recombinant enzyme
recombinant enzyme from Escherichia coli strain BL21(DE3) by hydrophobic interaction and anion-exchange chromatography, gel filtration, and ultrafiltration
recombinant enzyme from Escherichia coli strain BL21(DE3)/pMB1978 by anion exchange and hydrophobic interaction chromatography, followed by gel filtration and dialysis to over 95% purity
recombinant enzymes Alr and DadX
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage
recombinant His-tagged NusA fusion enzyme from Escherichia coli strain HMS174(lambdaDE3) by nickel affinity chromatography, ultrafiltration, cleavage of the fusion protein, and anion exchange chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, dialysis, and ultrafiltration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlusTM(DE3)-RIPL by nickel affinity chromatography, dialysis, and ultrafiltration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration to apparent homogeneity
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinit chromatography and gel filtration
recombinant His7-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatographyy
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 Star (DE3)pLysS by nickel affinity chromatography
recombinant protein
recombinant wild-type and mutant enzymes
Alkalihalophilus pseudofirmus
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and gel filtration