5.1.1.1: alanine racemase
This is an abbreviated version!
For detailed information about alanine racemase, go to the full flat file.
Word Map on EC 5.1.1.1
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5.1.1.1
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pyridoxal
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5'-phosphate
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peptidoglycan
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racemization
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d-cycloserine
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stearothermophilus
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plp-dependent
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d-amino
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aldimine
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d-alanyl-d-alanine
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exosporium
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pyridoxal-5'-phosphate-dependent
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drug development
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5'-phosphate-dependent
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d-alanine:d-alanine
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medicine
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biotechnology
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pharmacology
- 5.1.1.1
- pyridoxal
- 5'-phosphate
- peptidoglycan
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racemization
- d-cycloserine
- stearothermophilus
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plp-dependent
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d-amino
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aldimine
- d-alanyl-d-alanine
- exosporium
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pyridoxal-5'-phosphate-dependent
- drug development
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5'-phosphate-dependent
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d-alanine:d-alanine
- medicine
- biotechnology
- pharmacology
Reaction
Synonyms
1SFT, AAR, alanine racemase, AlaR, ALR, alr-2, ALR1, ALR2, Alr2 racemase, AlrA, AlrAba, AlrBax, AlrMtb, alrTt, ARL, BA0252, BAS0238, CBL/ALR, CdAlr, cystathionine beta-lyase, D-alanine racemase, DadB, DadX, dadXOF4, dal1, EcAlr, EcCBL, EfAlaR, L-Alanine racemase, L-Alanine:D-alanine racemase, MBalr1, MBAlr2, MetC, More, MurI, OEOE_1641, PDB, Racemase, alanine, tAlaRac, TmCBL, wMelCBL
ECTree
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KM Value
KM Value on EC 5.1.1.1 - alanine racemase
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18
D-Lysine
pH 8.0, 30°C, recombinant mutant I222T/Y354W
32
L-lysine
pH 8.0, 30°C, recombinant mutant I222T/Y354W
0.304
D-alanine
+/-0.034, Alr P219A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
0.311
D-alanine
+/-0.008, Alr wildtype, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
0.402
D-alanine
+/-0.055, Alr E221K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
0.439
D-alanine
+/-0.080, Alr E221A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
0.513
D-alanine
+/-0.084, Alr E221P, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
0.528
D-alanine
+/-0.079, Alr E165K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
0.592
D-alanine
+/-0.085, Alr E165A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
0.604
D-alanine
+/-0.070, Alr D164K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
0.615
D-alanine
+/-0.032, Alr D164A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
0.89
D-alanine
pH not specified in the publication, 30°C, recombinant enzyme
1.008
D-alanine
+/-0.069, Alr wildtype, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
2.8
D-alanine
pH 8.0, 30°C, recombinant wild-type enzyme
4
D-alanine
pH 8.0, 30°C, recombinant mutant I222T/Y354W
5.4
D-alanine
pH 8.5, temperature not specified in the publication, recombinant mutant K271T
6.1
D-alanine
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in 50 mM potassium phosphate buffer pH 7.4, at 30°C
7
D-alanine
pH 8.5, temperature not specified in the publication, recombinant wild-type enzyme
15.36
D-alanine
recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication
20.16
D-alanine
pH 11.0, 70°C, recombinant enzyme
20.78
D-alanine
Alkalihalophilus pseudofirmus
pH 10.5, 40°C, recombinant wild-type enzyme
52
D-alanine
pH 10.0, 65°C, recombinant mutant Q360Y
58.5
D-alanine
pH 10.0, 65°C, recombinant mutant S173D/Q360Y
110.3
D-alanine
pH 10.0, 65°C, recombinant mutant Q360I
160.9
D-alanine
pH 10.0, 65°C, recombinant mutant S173D
216.1
D-alanine
pH 10.0, 65°C, recombinant mutant Q360W
381.2
D-alanine
pH 10.0, 65°C, recombinant wild-type enzyme
0.38
L-alanine
recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer
1.049
L-alanine
+/-0.131, Alr P219A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
1.401
L-alanine
+/-0.209, Alr E221P, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
1.516
L-alanine
+/-0.083, Alr E221A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
1.562
L-alanine
+/-0.256, Alr E165A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
1.993
L-alanine
+/-0.269, Alr E221K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
2.057
L-alanine
+/-0.038, Alr E165K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
2.77
L-alanine
pH not specified in the publication, 30°C, recombinant enzyme
3.03
L-alanine
+/-0.114, Alr D164A, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
3.603
L-alanine
+/-0.180, Alr D164K, 30°C, pH 8.0, spectrophotometrically measured at 340 nm
4.2
L-alanine
recombinant His-tagged enzyme, pH 8.0, 37°C, Tris buffer
5.1
L-alanine
pH 8.0, 30°C, recombinant wild-type enzyme
8.2
L-alanine
recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer
8.3
L-alanine
pH 8.0, 30°C, recombinant mutant I222T/Y354W
9.3
L-alanine
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in 50 mM potassium phosphate buffer pH 7.4, at 30°C
12
L-alanine
recombinant MBP-tagged enzyme, pH 8.0, 37°C
13.8
L-alanine
pH 8.5, temperature not specified in the publication, recombinant mutant K271T
17.6
L-alanine
pH 8.5, temperature not specified in the publication, recombinant wild-type enzyme
35.9
L-alanine
recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication
56.17
L-alanine
Alkalihalophilus pseudofirmus
pH 10.5, 40°C, recombinant wild-type enzyme
83.1
L-alanine
pH 10.0, 65°C, recombinant mutant S173D/Q360Y
87.8
L-alanine
pH 10.0, 65°C, recombinant mutant Q360Y
136
L-alanine
pH 10.0, 65°C, recombinant mutant Q360I
157.5
L-alanine
pH 10.0, 65°C, recombinant mutant S173D
176.1
L-alanine
pH 10.0, 65°C, recombinant mutant Q360W
780.2
L-alanine
pH 10.0, 65°C, recombinant wild-type enzyme
additional information
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Km values of L-Ala in the presence of urea at various concentrations
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additional information
additional information
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Km values of L-Ala in the presence of urea at various concentrations
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additional information
additional information
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effect of NaCl on Km-value
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
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Vmax 110 micromol/min/mg D-alanine
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additional information
additional information
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Vmax 306 U/mg: D-alanine to L-alanine
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additional information
additional information
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Vmax 323 micromol/min/mg L-alanine
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additional information
additional information
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Vmax 345 U/mg: L-alanine to D-alanine
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additional information
additional information
Vmax for the racemization (D- to L-alanine and L- to D-alanine) is 87.0 and 84.8 U/mg, respectively.
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additional information
additional information
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Vmax for the racemization (D- to L-alanine and L- to D-alanine) is 87.0 and 84.8 U/mg, respectively.
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additional information
additional information
Michaelis-Menten steady-state kinetics
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additional information
additional information
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Michaelis-Menten steady-state kinetics
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additional information
additional information
Michaelis-Menten steady-state kinetics
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additional information
additional information
Michaelis-Menten steady-state kinetics
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additional information
additional information
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Michaelis-Menten steady-state kinetics
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