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4.99.1.8: heme ligase

This is an abbreviated version!
For detailed information about heme ligase, go to the full flat file.

Word Map on EC 4.99.1.8

Reaction

2 ferriprotoporphyrin IX =

beta-hematin

Synonyms

alpha-HDP, HDP, heme detoxification protein, heme-detoxification protein

ECTree

     4 Lyases
         4.99 Other lyases
             4.99.1 Sole sub-subclass for lyases that do not belong in the other subclasses
                4.99.1.8 heme ligase

Engineering

Engineering on EC 4.99.1.8 - heme ligase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H122A
the mutant shows less than half of the wild type activity
H172A
the mutant shows 44% of the wild type activity
H175A
the mutant shows less than half of the wild type activity
H192A
the mutant shows decreased hemozoin formation activity compared to the wild type enzyme
H197A
the mutant shows less than half of the wild type activity
H44A
the mutant shows decreased hemozoin formation activity compared to the wild type enzyme
H44A/H58A/H70A/H79A/H122A/H172A/H175A/H192A/H197A
the mutant shows less than half of the wild type activity
H58A
the mutant shows decreased hemozoin formation activity compared to the wild type enzyme
H70A
the mutant shows decreased hemozoin formation activity compared to the wild type enzyme
H79A
the mutant shows decreased hemozoin formation activity compared to the wild type enzyme
additional information
-
the truncated enzyme proteins HDP3 (encoded by amino acids 88-205 of the full-length protein, representing the fasciclin domain) and HDP2 (encoded by amino acids 1–87 and lacking the fasciclin domain) are unable to produce hemozoin. The full-length enzyme is required for heme binding and hemozoin production activities of the protein