4.99.1.3: sirohydrochlorin cobaltochelatase

This is an abbreviated version!
For detailed information about sirohydrochlorin cobaltochelatase, go to the full flat file.

Word Map on EC 4.99.1.3

4.99.1.3

protoporphyrin

megaterium

typhimurium

chlorophyll

precorrin-2

uroporphyrinogen

tetrapyrrole

primordial

sirohaem

dehydrogenation

histidine-rich

epr

atp-independent

ferrochelation

Reaction

+ 2 H+ =

Synonyms

anaerobic cobalt chelatase, archaeal cobaltochelatase, CbiK, CbiKc, CbiKp, CbiX, CbiX0H, CbiXL, CbiXOH, CbiXS, cobalamin cobalt chelatase, cobalt chelatase, cobaltochelatase, More, sirohydrochlorin cobalt chelatase, sirohydrochlorin cobalt-lyase, sirohydrochlorin cobaltochelatase, sirohydrochlorin-ferrochelatase

ECTree

4 Lyases

4.99 Other lyases

4.99.1 Sole sub-subclass for lyases that do not belong in the other subclasses

4.99.1.3 sirohydrochlorin cobaltochelatase

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Results	in table
3327	AA Sequence
1	CAS Registry Number
16	Cloned(Commentary)
4	Cofactor
7	Crystallization (Commentary)
2	Expression
3	General Information
2	KM Value [mM]
12	Localization
8	Metals/Ions
26	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
49	Organism
1	Oxidation Stability
5	Pathway
34	PDB ID
6	Posttranslational Modification
32	Protein Variants
13	Purification (Commentary)
1	Reaction
37	Reference
24	Specific Activity [micromol/min/mg]
70	Substrates and Products (Substrate)
12	Subunits
90	Synonyms
1	Systematic Name

Purification

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Purification on EC 4.99.1.3 - sirohydrochlorin cobaltochelatase

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6 entries

by using a metal chelating affinity column charged with Ni2+ or a chelating Sepharose-column charged with Co2+

Archaeoglobus fulgidus

O29537

681658

by using metal chelate affinity chromatography

Methanosarcina barkeri

P61816

682635

Ni-Sepharose column chromatography

Paracoccus pantotrophus

730419

purified anearobically

Priestia megaterium

649818

recombinant His-tagged wild-type isozyme and mutant DELTA28DVU0650 from Escherichia coli by nickel affinity chromatography, dialysis, anion exchange chromatography and gel filtration

Desulfovibrio vulgaris

Q72CB8, Q72EC8

690966

recombinant His-tagged wild-type isozyme from Escherichia coli by nickel affinity chromatography, dialysis, anion exchange chromatography and gel filtration

Desulfovibrio vulgaris

Q72CB8, Q72EC8

690966