4.99.1.2: alkylmercury lyase

This is an abbreviated version!
For detailed information about alkylmercury lyase, go to the full flat file.

Reaction

Synonyms

alkylmercury mercuric-lyase, lyase, alkylmercury, merB, MerB1, MerB2, MerB3, organomercurial lyase, organomercury lyase

ECTree

     4 Lyases

         4.99 Other lyases

             4.99.1 Sole sub-subclass for lyases that do not belong in the other subclasses

                4.99.1.2 alkylmercury lyase

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Results	in table
2	Activating Compound
711	AA Sequence
7	Application
1	CAS Registry Number
15	Cloned(Commentary)
4	Cofactor
5	Crystallization (Commentary)
10	General Information
1	General Stability
9	Inhibitors
17	KM Value [mM]
2	Localization
3	Metals/Ions
4	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
63	Organism
3	Pathway
33	PDB ID
2	pH Optimum
1	pH Range
9	Protein Variants
6	Purification (Commentary)
6	Reaction
5	Reaction Type
54	Reference
3	Specific Activity [micromol/min/mg]
1	Storage Stability
122	Substrates and Products (Substrate)
2	Subunits
67	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
7	Turnover Number [1/s]

Engineering

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Engineering on EC 4.99.1.2 - alkylmercury lyase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

C159S

Escherichia coli

P77072

site-directed mutagenesis, inactive mutant

698997

C160S

Escherichia coli

P77072

site-directed mutagenesis, mutant enzyme with moderately reduced activity

698997

C96S

Escherichia coli

P77072

site-directed mutagenesis, inactive mutant

698997

D99N

Escherichia coli

P77072

Cu(II) is bound to the active site cysteine residues

747098

D99S

Escherichia coli

P77072

mutation mimicks the sequence of Bacillus megaterium MerB. Cu(II) is bound to the active site cysteine residues and is displaced following the addition of either an organomercurial substrate or an ionic mercury product

747098

C160S

Serratia marcescens

-

50% inhibition by dithiothreitol in 20 mM cysteine

650094

additional information

3 entries

additional information

Escherichia coli

-

coexpression of enzyme and mercuric reductase in Arabidopsis thaliana leads to growth on 50fold higher methylmercury concentrations than wild type plants

653291

additional information

Escherichia coli

-

engineering of enzyme as to be targeted for accumulation in the endoplasmic reticulum and for secretion to the cell wall rendering the expressing plants resistant to organic mercury

653539

additional information

Pseudomonas sp. K-62

O07303

The organomercurial lyase gene of plasmid pMR26 (GenBank/DDBJ accession no. D83080) is amplified by PCR using two specific primers. The pBMK01 plasmid is transformed into Agrobacterium tumefaciens LBA4404 by electroporation, and its sequence is confirmed. The ppk/merT-transgenic tobacco (T3) is then transformed with this Agrobacterium tumefaciens, the tobacco callus shows more resistance to CH3Hg+ and accumulated significantly more mercury from CH3Hg+-containing medium than does the ppk/merT-transgenic and wild-type tobaccos

701887