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4.6.1.2: guanylate cyclase

This is an abbreviated version!
For detailed information about guanylate cyclase, go to the full flat file.

Word Map on EC 4.6.1.2

Reaction

GTP
=
3',5'-cyclic GMP
 +
diphosphate

Synonyms

alpha1 sGC, alpha1 soluble guanylyl cyclase, alpha1beta1 guanylyl cyclase, alpha1beta1 sGC, ANF-RGC, ANPRA, ANPRB, atrial natriuretic factor receptor guanylate cyclase, Atrial natriuretic peptide A-type receptor, Atrial natriuretic peptide B-type receptor, atrial natriuretic peptide receptor 2, BERhGC, BR1-GC, brassinosteroid receptor 1, CaRhGC, CNP-sensitive guanyly cyclase, CNP-sensitive guanylyl cyclase, CsGC-YO1, GC-A, GC-A receptor, GC-B, GC-C, GC-D, GC-G, GC1, GC2, GCA, GCC, gcy-18, gcy-23, GCY-35, gcy-8, GTP-pyrophosphate liase cyclizing, GTP-pyrophosphate lyase, guanyl cyclase, guanyl cyclase B, Guanylate cyclase, guanylate cyclase 1, guanylate cyclase 2, Guanylate cyclase 2D, retinal, Guanylate cyclase 2E, Guanylate cyclase 2F, retinal, guanylate cyclase A, guanylate cyclase B, guanylate cyclase C, Guanylate cyclase, olfactory, guanylyl cyclase, guanylyl cyclase A, guanylyl cyclase B, guanylyl cyclase C, guanylyl cyclase C receptor, guanylyl cyclase-A, guanylyl cyclase-A receptor, guanylyl cyclase-C, guanylyl cyclase-D, guanylyl cyclase-G, Gyc-88E, Gyc-89Da, Gyc-89Db, Gyca-99B, Gycb-100B, H-NOX, hSTAR, Intestinal guanylate cyclase, KSGC, L1 H-NOX, L2 H-NOX, membrane bound guanylate cyclase, membrane bound guanylyl cyclase, membrane guanylate cyclase, membrane guanylyl cyclase receptor, membrane-bound GC, membrane-bound guanylate cyclase, membrane-bound guanylyl cyclase, mGc, More, natriuretic peptide-activated guanylate cyclase, nitric oxid sensitive guanylyl cyclase, nitric oxide sensitive guanylyl cyclase, nitric oxide sensitive-guanylyl cyclase, nitric oxide-sensitive guanylate cyclase, nitric-oxide-sensitive guanylyl cyclase, NO receptor, NO receptor soluble guanylyl cyclase, NO sensitive guanylyl cyclase, NO- and haem-independent sGC, NO- and haem-independent soluble guanylate cyclase, NO-GC, NO-GC1, NO-GC2, NO-independent, heme-dependent soluble guanylate cyclase, NO-sensitive GC, NO-sensitive guanylyl cyclase, NO-sGC, NOGC1, NOsGC, NP-GC, NPR A, NPR-A, NPR-B, NPR1, NPR2, ONE-GC, ONE-GC membrane guanylate cyclase, Pac2, Pac3, particulate GC, particulate guanylate cyclase, particulate guanylyl cyclase, PepR1-GC, peptide hormone receptor guanylyl cyclase-C, PGC, photoreceptor guanylyl cyclase, phytosulfokine receptor, PSK receptor, PSKR1, ratGC, receptor guanylyl cyclase, receptor-type guanylyl cyclase, RET-GC1, retGC, RetGC1, RetGC2, retinal guanylate cyclase, retinal guanylyl cyclase, retinal membrane guanylyl cyclase, rGC, rhodopsin-guanylyl cyclase, Rod outer segment membrane guanylate cyclase, rod outer segment membrane guanylate cyclase type 1, ROS-GC, ROS-GC1, ROS-GC2, sGC, sGC alpha2 inhibitory isoform, sGC alpha2 isoform, sGCalpha1, sGCalpha2beta1, sGCbeta1, sGCbeta3, soluble GC, soluble guanylate cyclase, soluble guanylate cyclase alpha1, soluble guanylate cyclase alpha2, soluble guanylyl cyclase, SONO, STA, STA receptor, Tar4 protein

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.2 guanylate cyclase

Purification

Purification on EC 4.6.1.2 - guanylate cyclase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography
by gel filtration
-
by gel filtration and on nickel-agarose column, at least 95% pure
-
by metal affinity chromatography followed by gel filtration
-
cobalt affinity column chromatography and Mono Q anion exchange column chromatography
-
fast method
-
from lung
-
from lung tissue
-
glutathione-Sepharose 4B resin column chromatography, Q-Sepharose column chromatography, and Talon resin column chromatography
-
Gyc-88E(1-597) purified to more than 95% purity, by gel filtration and on anion-exchange column
-
native soluble guanylate cyclase from lung to high yield by two different steps of anion chromatography, hydroxyapatite chromatography, a third anion exchange chromatography, GTP affinity chromatography, and gel filtration
Ni-NTA column chromatography
-
Ni-NTA column chromatography, Ni-IDA column chromatography, and Superdex 75 gel filtration
Ni-NTA column chromatography, Q-Sepharose column chromatography, and Superdex 75 gel filtration
nickel affinity column chromatography
-
nickel HisTrap affinity column chromatography, HiTrap column chromatography, and Superdex 200 or Superdex 75 gel filtration
on Ni-NTA affinity column
-
on Ni2+ column and anion-exchange column
-
partial
phosphorylated form
-
preparation of GC-C in membranes isolated from mouse colon
-
Q-Sepharose, Blue-Sepharose, hydroxyapatite, gel filtration
-
recombinant GC-D partially from HEK-293T cell membranes, recombinant His-tagged intracellular cyclase domain of GC-D consisting of GC-D residues 850-1110 in insect HiFive cells by nickel affinity chromatography
-
recombinant GST-tagged retGC from COS-7 cells by glutathione affinity chromatography
recombinant His-tagged enzyme
-
recombinant His-tagged heterodimeric full-length and N-terminal fragments of sGC from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant intracellular domain of GCC
-
recombinant sGC
recombinant sGC, ion-exchange, hydroxyapatite, gel filtration
-
recombinant soluble guanylate cyclase from Spodoptera frugiperda SF9 cells by anion exchange chromatography, nickel affinity chromatography, and again anion exchange chromatography on a porus resin
-
recombinant Trx-tagged miniGC-C, comprising the extracellular domain, from Escherichia coli strain AD494(DE3) by Co2+ affinity chromatography, dialysis and ultrafiltration, cleavage of the thioredoxin tag
Strep-Tactin1 Superflow column chromatography, Mono Q column chromatography
-
truncated His-tagged guanylyl cyclase