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4.6.1.2: guanylate cyclase

This is an abbreviated version!
For detailed information about guanylate cyclase, go to the full flat file.

Word Map on EC 4.6.1.2

Reaction

GTP
=
3',5'-cyclic GMP
+
diphosphate

Synonyms

alpha1 sGC, alpha1 soluble guanylyl cyclase, alpha1beta1 guanylyl cyclase, alpha1beta1 sGC, ANF-RGC, ANPRA, ANPRB, atrial natriuretic factor receptor guanylate cyclase, Atrial natriuretic peptide A-type receptor, Atrial natriuretic peptide B-type receptor, atrial natriuretic peptide receptor 2, BERhGC, BR1-GC, brassinosteroid receptor 1, CaRhGC, CNP-sensitive guanyly cyclase, CNP-sensitive guanylyl cyclase, CsGC-YO1, GC-A, GC-A receptor, GC-B, GC-C, GC-D, GC-G, GC1, GC2, GCA, GCC, gcy-18, gcy-23, GCY-35, gcy-8, GTP-pyrophosphate liase cyclizing, GTP-pyrophosphate lyase, guanyl cyclase, guanyl cyclase B, Guanylate cyclase, guanylate cyclase 1, guanylate cyclase 2, Guanylate cyclase 2D, retinal, Guanylate cyclase 2E, Guanylate cyclase 2F, retinal, guanylate cyclase A, guanylate cyclase B, guanylate cyclase C, Guanylate cyclase, olfactory, guanylyl cyclase, guanylyl cyclase A, guanylyl cyclase B, guanylyl cyclase C, guanylyl cyclase C receptor, guanylyl cyclase-A, guanylyl cyclase-A receptor, guanylyl cyclase-C, guanylyl cyclase-D, guanylyl cyclase-G, Gyc-88E, Gyc-89Da, Gyc-89Db, Gyca-99B, Gycb-100B, H-NOX, hSTAR, Intestinal guanylate cyclase, KSGC, L1 H-NOX, L2 H-NOX, membrane bound guanylate cyclase, membrane bound guanylyl cyclase, membrane guanylate cyclase, membrane guanylyl cyclase receptor, membrane-bound GC, membrane-bound guanylate cyclase, membrane-bound guanylyl cyclase, mGc, More, natriuretic peptide-activated guanylate cyclase, nitric oxid sensitive guanylyl cyclase, nitric oxide sensitive guanylyl cyclase, nitric oxide sensitive-guanylyl cyclase, nitric oxide-sensitive guanylate cyclase, nitric-oxide-sensitive guanylyl cyclase, NO receptor, NO receptor soluble guanylyl cyclase, NO sensitive guanylyl cyclase, NO- and haem-independent sGC, NO- and haem-independent soluble guanylate cyclase, NO-GC, NO-GC1, NO-GC2, NO-independent, heme-dependent soluble guanylate cyclase, NO-sensitive GC, NO-sensitive guanylyl cyclase, NO-sGC, NOGC1, NOsGC, NP-GC, NPR A, NPR-A, NPR-B, NPR1, NPR2, ONE-GC, ONE-GC membrane guanylate cyclase, Pac2, Pac3, particulate GC, particulate guanylate cyclase, particulate guanylyl cyclase, PepR1-GC, peptide hormone receptor guanylyl cyclase-C, PGC, photoreceptor guanylyl cyclase, phytosulfokine receptor, PSK receptor, PSKR1, ratGC, receptor guanylyl cyclase, receptor-type guanylyl cyclase, RET-GC1, retGC, RetGC1, RetGC2, retinal guanylate cyclase, retinal guanylyl cyclase, retinal membrane guanylyl cyclase, rGC, rhodopsin-guanylyl cyclase, Rod outer segment membrane guanylate cyclase, rod outer segment membrane guanylate cyclase type 1, ROS-GC, ROS-GC1, ROS-GC2, sGC, sGC alpha2 inhibitory isoform, sGC alpha2 isoform, sGCalpha1, sGCalpha2beta1, sGCbeta1, sGCbeta3, soluble GC, soluble guanylate cyclase, soluble guanylate cyclase alpha1, soluble guanylate cyclase alpha2, soluble guanylyl cyclase, SONO, STA, STA receptor, Tar4 protein

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.2 guanylate cyclase

Cloned

Cloned on EC 4.6.1.2 - guanylate cyclase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
beta1 (residues 1-194) and beta2 (residues 1-217) homodimers expressed in Escherichia coli
-
by baculovirus expression system
-
catalytic domains (alphacat and betacat) of alpha1beta1 soluble guanylate cyclase are expressed in Escherichia coli
-
cloning of isozyme GC-A, DNA and amino acid sequence determination, analysis, and quantitative expression analysis, expression of FLAG-tagged or HA-tagged wild-type GC-A and of mutant GC-ADELTALys314-Gln330 and in HEK-293 cells
construction of a fluorescent alpha1/beta1-sGC heterodimer by fusion of the N-terminal YFP fragment and the C-terminal fragment to the N terminus and the C terminus of the alpha1- and beta1-subunit, respectively. Generated fusion proteins, wild-type and deletion mutants transiently expressed in a cGMP reporter cell line based on a Chinese hamster ovary cell line stably transfected with the cyclic nucleotide gated olfactory CNG2A-channel and cytosolic aequorin
-
construction of multiple N- and C-terminal deletion variants and cotransfecting them with full-length alpha1 subunit into COS cells
-
COS-7 cells transfected with the wild-type ONE-GC cDNA or its deletion mutants, ONE-GC mutants into pET30aLIC vector, expressed in Escherichia coli BL21-Codon-Plus-RIL
-
enzyme expression in heart tissue and heterogeneous expression patterns of NO-related regulatory enzyme systems, overview
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 (DE3) as a fusion protein with an N-terminal polyhistidine tag after subcloning into bacterial expression vector pET16b
expressed in Escherichia coli BL21 Star (pLysS) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) CodonPlus cells
-
expressed in Escherichia coli BL21(DE3)-R3 cells
expressed in HEK-293T cells
-
expressed in Sf21 insect cells
-
expressed in Sf9 insect cells
expression analysis of enzyme subunits during the estrous cycle overview
-
expression analysis of sGC subunits in pulmonary artery tissue from healthy and hypertensive lungs
expression in CHO cells in a reporter-coupled system, overview
-
expression in COS-7 cells
-
expression in Escherichia coli
expression in HEK-293 cell
expression in in HEK-293T cells
-
expression in Sf9 insect cells
-
expression of a Trx-tagged miniGC-C, comprising the extracellular domain, in Escherichia coli strain AD494(DE3) using expression vector pET-32a
expression of alpha1 and beta1 subunits and mutant enzyme in COSm6 and A7r5 smooth muscle cells
-
expression of alpha1 and beta1 subunits in Sf9 insect cells
-
expression of alpha1 and beta1 subunits of sGC in Sf9 insect cells
expression of His-tagged heterodimeric full-length and N-terminal fragments of Manduca sexta sGC in Escherichia coli strains BL21(DE3)
expression of His-tagged soluble guanylate cyclase in Spodoptera frugiperda SF9 cells using the baculovirus transfection method
-
expression of His-tagged truncated cyclase domain in Escherichia coli C41. The mutant enzyme E497K/C566D is expressed the N-terminal YFP-tagged constructs in oocytes
expression of isoform alpha2,beta1 in Sf9 cells
expression of sGC in BE2 human neuroblastoma cell line and in Spodoptera frugiperda SF9 cells, quantitative expression analysis of sGC splice variants, overview
expression of sGC in insect cells
-
expression of sGC in Sf9 insect cells and COS-7 cells
-
expression of sGCalpha and sGCbeta subunit in Sf1 insect cells
-
expression of the heme-free alpha1-subunit/H105F beta1-subunit sGC mutant in COS-7 cells
-
expression of the His6-tagged truncated enzyme mutant in CT26 mouse colon cancer cells, subclining in 293T cells
expression of the intracellular domain of GCC in Sf21 insect cells
-
expression of wild-type alpha1beta2 sGC enzyme in Spodoptera frugiperda Sf9 cells, and of heme domain mutants in Escherichia coli
-
expression of wild-type and C122A mutant enzymes in COS-7 cells
-
expression of wild-type and mutant enzymes in COS-7 cells
-
expression of wild-type and mutant in Sf9 cells
-
expression of wild-type GFP-tagged RetGC1 and commercially available dsRet-tagged RetGC1 in HEK-293 cells, co-expression with either fluorescently tagged or non-tagged guanylyl cyclase activating protein 1, with inactivated metal binding in individual EF-hands, in cell membranes. The uniform cellular distribution of GCAP1, also in the nucleus, drastically changes when the cells express both GCAP1-GFP and RetGC1, GCAP1 is then depleted from the nuclei and only observed in the cytoplasm of the cells, overview
-
full-length genomic gcy gene sequence fused to the GFP gene and injected into wild-type animals
functional chimeras between the catalytic domains of the mycobacterial adenylyl cyclase Rv1625c and a Paramecium guanylyl cyclase
-
functional transient expression of GC-D HEK-293T cell membranes, expression of His-tagged intracellular cyclase domain of GC-D consisting of GC-D residues 850-1110 in insect HiFive cells by using the baculovirus expression system
-
GC-A gene, genotyping of hypertensive and normotensive Japanese, association of CT dinucleotide repeat polymorphism in the 5'-flanking region of the GC-A gene with essential hypertension in the Japanese, overview
-
gene CsGC-YO1, DNA and amino acid sequence determination and analysis, quantitative expression by real-time PCR assay, expression of the extracellular domain of CsGC-YO1 in Escherichia coli
gene CsGC-YO1, DNA and amino acid sequence determination and analysis, tissue expression analysis, expression of the extracellular domain of CsGC-YO1 in Escherichia coli
gene CYG12 encodes the soluble isozyme
gene encoding isozyme Gyc-89Da, expression analysis and promoter analysis, expression in Saccharomces cerevisiae
gene encoding isozyme Gyc-89Db, expression analysis and promoter analysis, expression in Saccharomces cerevisiae
gene gc1, DNA and amino acid sequence determination, RET-GC1 expression analysis in retinal tissue, overview
gene gc2, DNA and amino acid sequence determination, RET-GC2 expression analysis in retinal tissue, overview
gene gc3, DNA and amino acid sequence determination, RET-GC3 expression analysis in retinal tissue, overview
genes gucy1a3 and gucy2F, DNA and amino acid sequence determination and analysis, and expression analysis. Overexpression of wild-type and mutant enzymes in zebrafish embryos
-
Gyc-88E(1-597) expressed in S2 cells as a polyoma-tagged construct under the control of the metallothionein promoter
-
into pET-20b vector
into the pCRHT7 TOPOH-NT vector and expressed in Escherichia coli BL21 (DE3) pLysS cells
-
isolation of two mRNA species for sGC beta2 with dissimilar 5'-untranslated regions from human kidney, translational mechanism of the sGC beta2-subunit, overview. Insertion of these regions between the two luciferase genes of a bicistronic vector and transfection into HeLa cells, both sGC beta2 leaders have internal ribosome entry site, IRES, activity in a cell-type dependent manner. The sGC beta2 IRES is functional in a wide range of cell lines, e.g. HeLa cells, Hep-G2 cells, COS-7 cells, and CCL-185 cells
isozyme sGCalpha1
-
isozymes GC-A to GC-G
-
isozymes GC-D, GC-E, to GC-G
MDCK cell lines expressing HA-tagged GCC or HA-tagged GCC lacking the amino acids from the conserved 63 amino acid span
-
membrane-bound guanylate cyclase, phylogenetic analysis
N-terminal heme-binding regions of subunit beta1 from soluble guanylate cyclase are generated by subcloning specific constructs into the Escherichia coli expression vector pET-20b. The shortest region that is subcloned, has heme-bound, and is expressed well is beta1(1-194)
-
overexpression in a baculovirus/Sf9 system
quantitative analysis of promoter activity and determination of transcriptional start sites within the 5'-flanking region of alpha1 and beta1 sGC using transiently transfected luciferase reporter constructs, expression in human aortic smooth muscle cells and in COS-7 cells
-
sGC beta1 cDNA inserted between the NotI and XbaI sites of the plasmid pFastBac1, H6sGC alpha1 gene inserted between the NotI and XbaI sites of pFastBac1, expression in Sf9 cells
-
the beta1 subunit of soluble guanylyl cyclase is expressed in Escherichia coli BL21 (DE3) pLysS cells
the sGCalpha1 gene encodes for an essential part of the catalytic domain. Quantitative expression analysis by RT-PCR and relative levels of alpha1, alpha2, and beta1 subunit mRNA in male KO tissues versus male wild-type tissues
-
transfection of COS-7 cell
transfection of HEK-293T cell
transfection of Sf9 cells
-
transient expression in COS-7 cells
transient expression in COS-7 cells as GST-tagged enzyme, reconstitution of the Galphat-retGC interaction in COS-7 cells, overview
transiently transfected into COS-7 cells
-
wild-type and mutant enzymes expressed in Sf21/baculovirus system
-
wild-type sGC and mutant sGC constructs fused at the C-terminus to GFP and expressed in gc-null cells