4.6.1.17: cyclic pyranopterin monophosphate synthase
This is an abbreviated version!
For detailed information about cyclic pyranopterin monophosphate synthase, go to the full flat file.
Reaction
Synonyms
cnx3, cyclic pyranopterin monophosphate synthase accessory protein, EC 4.1.99.18, MoaC, MoaC2, Moco-biosynthesis protein, MOCS1B, molybdenum cofactor biosynthesis protein C, ST0472
ECTree
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Crystallization
Crystallization on EC 4.6.1.17 - cyclic pyranopterin monophosphate synthase
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purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.003 ml of 10.5 mg/ml protein solution with 0.0015 ml reservoir solution containing 0.025 M potassium sodium tartrate tetrahydrate, pH 8.0, and equilibration against 1 ml reservoir solution, 15 days, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement calculations
purified recombinant MoaC2s in apo form, X-ray diffraction structure determination and analysis at 2.2-2.5 A resolution
sitting-drop method, hanging-drop vapour-diffusion method, at room temperature