4.6.1.14: glycosylphosphatidylinositol diacylglycerol-lyase

This is an abbreviated version!
For detailed information about glycosylphosphatidylinositol diacylglycerol-lyase, go to the full flat file.

Reaction

Synonyms

(glycosyl)phosphatidylinositol-specific phospholipase C, EC 3.1.4.47, GDE3, glycan-phosphatidylinositol-specific phospholipase C, glycosyl inositol phospholipid anchor-hydrolyzing enzyme, glycosylphosphatidylinositol phospholipase C, glycosylphosphatidylinositol-phospholipase C, glycosylphosphatidylinositol-specific phospholipase C, glycosylphosphatidylinositol-specific phospholipase D, GPI-PC, GPI-phosphatidylinositol-phospholipase C, GPI-phospholipase C, GPI-phospholipase C polypeptide, GPI-PLC, GPI-PLC enzyme, GPI-PLCp, GPI-PLD, GPI-specific phospholipase C, phospholipase C, glycosylphosphatidylinositol, variant-surface-glycoprotein phospholipase C, variant-surface-glycoprotein-1,2-didecanoyl-sn-phosphatidylinositol inositolphosphohydrolase, VSG-lipase

ECTree

     4 Lyases

         4.6 Phosphorus-oxygen lyases

             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)

                4.6.1.14 glycosylphosphatidylinositol diacylglycerol-lyase

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Results	in table
15	Activating Compound
22	AA Sequence
3	Application
1	CAS Registry Number
10	Cloned(Commentary)
13	Disease/ Diagnostics
2	Expression
7	General Information
3	General Stability
53	Inhibitors
1	Ki Value [mM]
10	KM Value [mM]
20	Localization
5	Metals/Ions
4	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
64	Organism
5	pH Optimum
3	pH Range
2	Posttranslational Modification
58	Protein Variants
6	Purification (Commentary)
1	Reaction
1	Reaction Type
37	Reference
14	Source Tissue
11	Specific Activity [micromol/min/mg]
3	Storage Stability
63	Substrates and Products (Substrate)
6	Subunits
48	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Substrates Products

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of the Enzyme Summary Page.

Substrates Products on EC 4.6.1.14 - glycosylphosphatidylinositol diacylglycerol-lyase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

1,2-dimyristoyl-sn-phosphatidylinositol + H2O

?

Trypanosoma brucei

-

poor substrate

648767

-

-

?

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol

6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol

4 entries

acetylcholinesterase

soluble acetylcholinesterase + 1,2-diacylglycerol

2 entries

dimyristyl-P + H2O

?

Trypanosoma brucei

-

-

135294

-

-

?

glycolipid A

?

2 entries

glycolipid Y

?

2 entries

glycosylated phosphatidylinositol + H2O

?

Trypanosoma brucei

-

-

135294

-

-

?

glycosylphosphatidylinositol-anchored protein

?

Mus musculus

-

-

715721

-

-

?

lipid A

?

Trypanosoma brucei

-

biological precursor of variant-surface-glycoprotein glycolipid

648767

-

-

?

membrane form variant surface glycoprotein + H2O

soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol

Trypanosoma brucei

-

mfVSG, enzyme catalyzes the conversion of membrane form variant surface glycoproteins to soluble variant surface glycoproteins, with the release of sn-1,2-dimyristylglycerol

135294

-

-

?

myristate-labeled variant-surface-glycoprotein

?

Trypanosoma brucei

-

-

648779

-

-

?

phosphatidylinositol

inositol 1,2-cyclic phosphate + inositol phosphate

5 entries

pronase fragment + H2O

?

Trypanosoma brucei

-

aspartylethanolamine-glycolipid moiety of mfVSG

135294

-

-

?

pronase fragment of mfVSG + H2O

?

Trypanosoma brucei

-

contains only the terminal amino acid and the glycolipid anchor

135294

-

-

?

variant surface glycoprotein

?

2 entries

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

13 entries

additional information

?

-

11 entries

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol

6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol

Bacillus cereus

-

-

661039

-

-

?

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol

6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol

Rattus norvegicus

-

-

661039

-

-

?

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol

6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol

Rattus norvegicus

-

the assay contains acetylcholinesterase

661039

-

-

?

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol

6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol

Trypanosoma brucei

-

-

661039, 662276, 662808

-

-

?

acetylcholinesterase

soluble acetylcholinesterase + 1,2-diacylglycerol

Arachis hypogaea

-

isolated from bovine erythrocytes, does not act on membrane-bound forms but hydrolyzes the membrane-anchor from solubilized substrate

648770

-

648770

?

acetylcholinesterase

soluble acetylcholinesterase + 1,2-diacylglycerol

Trypanosoma brucei

-

-

648778

-

-

?

glycolipid A

?

Trypanosoma brucei

-

is cleaved when Trypanosoma brucei encounters a mildly alkaline or hypotonic extracellular environment

679738

-

-

?

glycolipid A

?

Trypanosoma brucei 427

-

is cleaved when Trypanosoma brucei encounters a mildly alkaline or hypotonic extracellular environment

679738

-

-

?

glycolipid Y

?

Trypanosoma brucei

-

-

679738

-

-

?

glycolipid Y

?

Trypanosoma brucei 427

-

-

679738

-

-

?

phosphatidylinositol

inositol 1,2-cyclic phosphate + inositol phosphate

Arachis hypogaea

-

-

648770

-

648770

?

phosphatidylinositol

inositol 1,2-cyclic phosphate + inositol phosphate

Arachis hypogaea

-

-

648776

-

-

?

phosphatidylinositol

inositol 1,2-cyclic phosphate + inositol phosphate

Trypanosoma brucei

-

-

648778, 648781

-

-

?

phosphatidylinositol

inositol 1,2-cyclic phosphate + inositol phosphate

Trypanosoma brucei

-

very poor substrate

648769

-

-

?

phosphatidylinositol

inositol 1,2-cyclic phosphate + inositol phosphate

Trypanosoma brucei

-

at 0.005 mM phosphatidylinositol, hydrolysis is maximal at 0.005% Triton X-100, at 1 mM phosphatidylinositol hydrolysis is maximal at 0.05% Triton X-100

648776

-

-

?

variant surface glycoprotein

?

Trypanosoma brucei

-

on exposure to mild alkali

679738

-

-

?

variant surface glycoprotein

?

Trypanosoma brucei 427

-

on exposure to mild alkali

679738

-

-

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Arachis hypogaea

-

-

648776

-

-

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Arachis hypogaea

-

peanut enzyme does not act on membrane-bound forms but hydrolyzes the membrane-anchor from solubilized substrate

648770

-

-

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Arachis hypogaea

-

isolated from Trypanosoma brucei

648770

-

-

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Mus musculus

-

highly specific

648768

-

-

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Mus musculus

-

isolated from Trypanosoma equiperdum BoTat-1

648768

-

-

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Rattus norvegicus

-

-

648771, 648772

-

-

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Saccharomyces cerevisiae

-

-

648771

-

-

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Trypanosoma brucei

-

-

648773, 648775, 648776, 648777, 648778, 648779, 648780, 648781, 648782, 648783

-

-

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Trypanosoma brucei

-

-

648769

i.e. 1,2-dimyristoylglycerol

648769

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Trypanosoma brucei

-

highly specific

648767

i.e. 1,2-dimyristoylglycerol

648767

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Trypanosoma brucei

-

substrate is tethered to cell membrane by glycolipid moiety containing 1,2-dimyristoyl-sn-phosphatidylinositol

648767

i.e. 1,2-dimyristoylglycerol

648767

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Trypanosoma brucei

-

enzyme activity is stimulated by acid-treatment of the cells

648774

-

-

?

variant-surface-glycoprotein

1,2-didecanoylglycerol + soluble variant-surface-glycoprotein

Trypanosoma brucei

-

phospholipase C-type hydrolysis

648767

i.e. 1,2-dimyristoylglycerol

648767

?

additional information

?

-

Arachis hypogaea

-

no substrates are phosphatidylcholine, phosphatidylserine, phosphatidylinositol 4-monophosphate, phosphatidylinositol 4,5-bisphosphate

648770

-

-

?

additional information

?

-

Trypanosoma brucei

-

glucosaminyl(alpha1-, 6)inositol is the crucial glycan moiety for substrate recognition

648769

-

-

?

additional information

?

-

Trypanosoma brucei

-

no substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidylinositol

648767

-

-

?

additional information

?

-

Trypanosoma brucei

-

does not digest glycolipid Z and glycolipid C

679738

-

-

?

additional information

?

-

Trypanosoma brucei

-

enzyme exhibits substantial specificity for molecules containing glycosylated phosphatidylinositol

135294

-

-

?

additional information

?

-

Trypanosoma brucei

-

GPI-PLC is a signalling enzyme, and DAG is a second messenger for GPI-PLC. Its enzyme activity is important for stimulating uptake of transferrin

690839

-

-

?

additional information

?

-

Trypanosoma brucei

-

[32P]phosphatidylinositol-4-phosphate, [32P]phosphatidylinositol 4,5-bisphosphate or [3H]phosphatidylcholine are not significantly hydrolyzed by the GPI-PLC

135294

-

-

?

additional information

?

-

Trypanosoma brucei

-

hippocampal detergent-resistant membranes: alpha2beta subunits of voltage-gated calcium channels, alpha2delta-1, alpha2delt-2, and alpha2delt-3, show all properties expected of GPI-anchored proteins, which are substrates for GPI-PLC

710429

-

-

?

additional information

?

-

Trypanosoma brucei 427

-

enzyme exhibits substantial specificity for molecules containing glycosylated phosphatidylinositol

135294

-

-

?

additional information

?

-

Trypanosoma brucei 427

-

[32P]phosphatidylinositol-4-phosphate, [32P]phosphatidylinositol 4,5-bisphosphate or [3H]phosphatidylcholine are not significantly hydrolyzed by the GPI-PLC

135294

-

-

?

additional information

?

-

Trypanosoma brucei 427

-

does not digest glycolipid Z and glycolipid C

679738

-

-

?