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C184S
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fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C24A
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exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
C24S
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fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C269-270-273S
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33% activity of wild-type enzyme
C269C/C270S/C273S
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exhibits activity in the range detected for the unmutated protein, localization in glycosome
C269S
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fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C269S/C270S/C273S
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exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
C270S
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fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C273S
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fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C332A
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exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
C332S
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fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C347S
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fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C80F
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enzyme is inactive
C80S
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fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C80T
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33% activity of wild-type enzyme, resistant to p-chloromercuriphenylsulfonic acid
H34Q
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enzyme is totally inactive
Q81A
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enzyme is inactive
Q81E
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enzyme is inactive
Q81G
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enzyme is inactive
Q81K
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enzyme is inactive
Q81N
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specific activity is 500fold decreased
C184A
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exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
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C347A
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can not exit glycosomes after treatment of cells expressing the protein with mild base or hypo-osmotic buffer
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C80A
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exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
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Q81L
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fails to cleave glycosylphosphatidylinositols despite being transported from glycosomes to the endoplasmic reticulum after hypoosmotic or mild alkaline treatment of parasites, lacks enzyme activity
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C184A
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activity is not effected
C184A
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exhibits activity in the range detected for the unmutated protein, deficiency of cell-associated gp63, localization in endo-lysosome
C184A
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exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
C269S/C270S
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exhibits activity in the range detected for the unmutated protein, localization in glycosome
C269S/C270S
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exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
C269S/C273S
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exhibits activity in the range detected for the unmutated protein, localization in glycosome
C269S/C273S
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exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
C270S/C273S
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exhibits activity in the range detected for the unmutated protein, localization in glycosome
C270S/C273S
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exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
C347A
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exhibits activity in the range detected for the unmutated protein, deficiency of cell-associated gp63, localization in endo-lysosome
C347A
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can not exit glycosomes after treatment of cells expressing the protein with mild base or hypo-osmotic buffer
C80A
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33% activity of wild-type enzyme, resistant to p-chloromercuriphenylsulfonic acid
C80A
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exhibits activity in the range detected for the unmutated protein, localization in glycosome
C80A
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exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
Q81L
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inactive
Q81L
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catalytically inactive mutant
Q81L
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enzyme is inactive
Q81L
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fails to cleave glycosylphosphatidylinositols despite being transported from glycosomes to the endoplasmic reticulum after hypoosmotic or mild alkaline treatment of parasites, lacks enzyme activity
additional information
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tetracyclin-inducible GPIPLC-gene, in conditional knock-out bloodstream forms and in procyclic cell line
additional information
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PLC elimination construct, which yielded in a PLC null-mutant
additional information
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PLC elimination construct, which yielded in a PLC null-mutant
additional information
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generation of conditional knock-out bloodstraem forms
additional information
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strain RUMP528, mutant GPI-PLC-/- cells, only 10% loss of alpha-toxin signal in cells exposed to hypo-osmotic buffer
additional information
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strain RUMP528, mutant GPI-PLC-/- cells, only 10% loss of alpha-toxin signal in cells exposed to hypo-osmotic buffer
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