Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.6.1.14: glycosylphosphatidylinositol diacylglycerol-lyase

This is an abbreviated version!
For detailed information about glycosylphosphatidylinositol diacylglycerol-lyase, go to the full flat file.

Word Map on EC 4.6.1.14

Reaction

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
=
6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate
+
1,2-diacyl-sn-glycerol

Synonyms

(glycosyl)phosphatidylinositol-specific phospholipase C, EC 3.1.4.47, GDE3, glycan-phosphatidylinositol-specific phospholipase C, glycosyl inositol phospholipid anchor-hydrolyzing enzyme, glycosylphosphatidylinositol phospholipase C, glycosylphosphatidylinositol-phospholipase C, glycosylphosphatidylinositol-specific phospholipase C, glycosylphosphatidylinositol-specific phospholipase D, GPI-PC, GPI-phosphatidylinositol-phospholipase C, GPI-phospholipase C, GPI-phospholipase C polypeptide, GPI-PLC, GPI-PLC enzyme, GPI-PLCp, GPI-PLD, GPI-specific phospholipase C, phospholipase C, glycosylphosphatidylinositol, variant-surface-glycoprotein phospholipase C, variant-surface-glycoprotein-1,2-didecanoyl-sn-phosphatidylinositol inositolphosphohydrolase, VSG-lipase

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.14 glycosylphosphatidylinositol diacylglycerol-lyase

Engineering

Engineering on EC 4.6.1.14 - glycosylphosphatidylinositol diacylglycerol-lyase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C184A
C184S
-
fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C24A
-
exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
C24S
-
fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C269-270-273S
-
33% activity of wild-type enzyme
C269C/C270S/C273S
-
exhibits activity in the range detected for the unmutated protein, localization in glycosome
C269S
-
fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C269S/C270S
C269S/C270S/C273S
-
exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
C269S/C273S
C270S
-
fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C270S/C273S
C273S
-
fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C332A
-
exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
C332S
-
fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C347A
C347S
-
fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C80F
-
enzyme is inactive
C80S
-
fully active in vitro and still susceptible to p-chloromercuriphenylsulfonic acid
C80T
-
33% activity of wild-type enzyme, resistant to p-chloromercuriphenylsulfonic acid
H34Q
-
enzyme is totally inactive
Q81A
-
enzyme is inactive
Q81E
-
enzyme is inactive
Q81G
-
enzyme is inactive
Q81K
-
enzyme is inactive
Q81N
-
specific activity is 500fold decreased
C184A
-
exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
-
C347A
-
can not exit glycosomes after treatment of cells expressing the protein with mild base or hypo-osmotic buffer
-
C80A
-
exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer
-
Q81L
-
fails to cleave glycosylphosphatidylinositols despite being transported from glycosomes to the endoplasmic reticulum after hypoosmotic or mild alkaline treatment of parasites, lacks enzyme activity
-
additional information