4.6.1.14: glycosylphosphatidylinositol diacylglycerol-lyase

This is an abbreviated version!
For detailed information about glycosylphosphatidylinositol diacylglycerol-lyase, go to the full flat file.

Word Map on EC 4.6.1.14

4.6.1.14

trypanosoma

brucei

glycosylphosphatidylinositols

gpi-anchored

procyclic

glycosylphosphatidylinositol-anchored

tsetse

gpi-deficient

medicine

anti-crd

stumpy

gpi-specific

Reaction

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol

6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate

Synonyms

(glycosyl)phosphatidylinositol-specific phospholipase C, EC 3.1.4.47, GDE3, glycan-phosphatidylinositol-specific phospholipase C, glycosyl inositol phospholipid anchor-hydrolyzing enzyme, glycosylphosphatidylinositol phospholipase C, glycosylphosphatidylinositol-phospholipase C, glycosylphosphatidylinositol-specific phospholipase C, glycosylphosphatidylinositol-specific phospholipase D, GPI-PC, GPI-phosphatidylinositol-phospholipase C, GPI-phospholipase C, GPI-phospholipase C polypeptide, GPI-PLC, GPI-PLC enzyme, GPI-PLCp, GPI-PLD, GPI-specific phospholipase C, phospholipase C, glycosylphosphatidylinositol, variant-surface-glycoprotein phospholipase C, variant-surface-glycoprotein-1,2-didecanoyl-sn-phosphatidylinositol inositolphosphohydrolase, VSG-lipase

ECTree

4 Lyases

4.6 Phosphorus-oxygen lyases

4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)

4.6.1.14 glycosylphosphatidylinositol diacylglycerol-lyase

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Results	in table
15	Activating Compound
22	AA Sequence
3	Application
1	CAS Registry Number
10	Cloned(Commentary)
13	Disease/ Diagnostics
2	Expression
7	General Information
3	General Stability
53	Inhibitors
1	Ki Value [mM]
10	KM Value [mM]
20	Localization
5	Metals/Ions
4	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
64	Organism
5	pH Optimum
3	pH Range
2	Posttranslational Modification
58	Protein Variants
6	Purification (Commentary)
1	Reaction
1	Reaction Type
37	Reference
14	Source Tissue
11	Specific Activity [micromol/min/mg]
3	Storage Stability
63	Substrates and Products (Substrate)
6	Subunits
48	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

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Cloned on EC 4.6.1.14 - glycosylphosphatidylinositol diacylglycerol-lyase

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expression in Escherichia coli

Trypanosoma brucei

648773, 648780, 648783

expression in Leishmania major

Trypanosoma brucei

662276

expression in Xenopus oocytes

Trypanosoma brucei

648783

expression of a teracycline-inducible GPIPLC-gene in conditional knock-out bloodstraem forms and in procyclic cell line

Trypanosoma brucei

648781

expression of the PLC elimination construct in Trypanosoma brucei

Trypanosoma brucei

648775, 648778

mutants subcloned into pLew82(DLUC) vector, Cys mutants stably expressed in strain RUMP528

Trypanosoma brucei

679738

overexpression in Escherichia coli

Trypanosoma brucei

648777