4.5.1.2: 3-chloro-D-alanine dehydrochlorinase

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For detailed information about 3-chloro-D-alanine dehydrochlorinase, go to the full flat file.

Reaction

Synonyms

3-chloro-D-alanine chloride-lyase, beta-chloro-D-alanine dehydrochlorinase, DCyD

ECTree

     4 Lyases

         4.5 Carbon-halide lyases

             4.5.1 Carbon-halide lyases (only sub-subclass identified to date)

                4.5.1.2 3-chloro-D-alanine dehydrochlorinase

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Results	in table
1	CAS Registry Number
1	Cloned(Commentary)
4	Cofactor
4	Crystallization (Commentary)
2	General Information
23	Inhibitors
9	KM Value [mM]
6	Localization
6	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
46	Organism
5	pH Optimum
1	pH Range
1	pH Stability
7	Protein Variants
3	Purification (Commentary)
1	Reaction
12	Reaction Type
16	Reference
4	Specific Activity [micromol/min/mg]
1	Storage Stability
57	Substrates and Products (Substrate)
5	Subunits
3	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Stability [°C]

Engineering

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Engineering on EC 4.5.1.2 - 3-chloro-D-alanine dehydrochlorinase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

H80Q

Salmonella enterica subsp. enterica serovar Typhimurium

-

site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme

730698

Q77H

Salmonella enterica subsp. enterica serovar Typhimurium

-

site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 30% activity with beta-chloro-D-alanine

730698

S78A

Salmonella enterica subsp. enterica serovar Typhimurium

-

site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 20% activity with beta-chloro-D-alanine

730698

T288E

Salmonella enterica subsp. enterica serovar Typhimurium

-

site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme

730698

T315L/T288E

Salmonella enterica subsp. enterica serovar Typhimurium

-

site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme

730698

Y261F

Salmonella enterica subsp. enterica serovar Typhimurium

-

site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme

730698

Y287F

Salmonella enterica subsp. enterica serovar Typhimurium

-

site-directed mutagenesis, inactive mutant with all substrates tested

730698