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4.4.1.9: L-3-cyanoalanine synthase

This is an abbreviated version!
For detailed information about L-3-cyanoalanine synthase, go to the full flat file.

Word Map on EC 4.4.1.9

Reaction

L-cysteine
+
hydrogen cyanide
=
L-3-cyanoalanine
+
hydrogen sulfide

Synonyms

AtcysC1, beta-CAS, beta-cyano-L-alanine synthase, beta-cyanoalanine synthase, beta-cyanoalanine synthetase, BsaS, Bsas3, BsasA, BsasB, CAS, CAS1, CAS2, cyanoalanine synthase, CYS-C1, L-cysteine hydrogen-sulfide-lyase, L-cysteine: hydrogen sulphide lyase, O-acetyl-L-serine sulfhydrylase, PCAS-1, PCAS-2, synthase,.beta.-cyanoalanine, Tu-CAS

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.9 L-3-cyanoalanine synthase

Cloned

Cloned on EC 4.4.1.9 - L-3-cyanoalanine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana and in Saccharomyces cerevisiae
-
expression in Escherichia coli
expression of PCAS-1 in Escherichia coli
expression of PCAS-2 in Escherichia coli
full-length MdCAS2 cDNA clones introduced into plasmid pMAL-c2X, expressed in Escherichia coli BL21 (DE3)
full-length pMdCAS1 cDNA clones introduced into plasmid pMAL-c2X, expressed in Escherichia coli BL21 (DE3)
gene Tu-CAS , DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli
recombinant enzyme expression as N-terminally His-tagged, functional dimeric type and mutant enzyme proteins lacking the mitochondrial localization sequence