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4.4.1.4: alliin lyase

This is an abbreviated version!
For detailed information about alliin lyase, go to the full flat file.

Word Map on EC 4.4.1.4

Reaction

an S-alkyl-L-cysteine S-oxide
=
an alkyl sulfenate
+
2-aminoacrylate

Synonyms

alkylcysteine sulfoxide lyase, alkylsulphenate lyase, ALL1, alliin lyase, alliin-lyase, alliinase, alliinase I, alliinase II, allinase, C-S lyase, C-S-lyase, cys sulfoxide lyase, cysteine sulfoxide lyase, Cysteine sulphoxide lyase, L-(+)-S-alk(en)ylcysteine sulfoxide lyase, L-cysteine sulfoxide lyase, lyase, alliin, More, P-19486 alliinase, S-alkyl(en)yl-L-cysteine lyase, S-alkylcysteine sulfoxide lyase

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.4 alliin lyase

Purification

Purification on EC 4.4.1.4 - alliin lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
alliin lyase I and alliin lyase II
-
lectin-alliinase complexes do not occur in vivo but are formed in vitro after homogenization of the tissue
native enzyme 300fold to homogeneity by anion exchange, hydrophobic interaction, and aminohexyl affinity chromatography, followed by another different step of anion exchange chromatography and gel filtration
-
native enzyme by gel filtration and lectin concanavalin A affinity chromatography
-
native enzyme from cloves by ammonium sulfate fractionation, dialysis, and gel filtration
-
precipitation with ammonium sulfate, ion-exchange, hydroxyapatite, and gel-filtration chromatographies in sequence
-