Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.4.1.30: phycobiliprotein beta-cysteine-155 phycobilin lyase

This is an abbreviated version!
For detailed information about phycobiliprotein beta-cysteine-155 phycobilin lyase, go to the full flat file.

Word Map on EC 4.4.1.30

Reaction

[C-phycocyanin beta-subunit]-Cys155-phycocyanobilin
=
apo-[C-phycocyanin beta-subunit]
+
(2R,3E)-phycocyanobilin

Synonyms

all5339, cpcT, CpcT1, CpeT, CpeT1, PhiCpeT, phycobiliprotein lyase CpcT

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.30 phycobiliprotein beta-cysteine-155 phycobilin lyase

Engineering

Engineering on EC 4.4.1.30 - phycobiliprotein beta-cysteine-155 phycobilin lyase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C116S
in vitro assay,11% of wild-type activity
C137S
in vitro assay, 11% of wild-type activity
D163A
lyase activity is less than 5% compared to wild-type enzyme
D163V
lyase activity is less than 5% compared to wild-type enzyme
D63N
lyase activity is about 70% compared to wild-type enzyme
E111Q
lyase activity is about 75% compared to wild-type enzyme
H33F
in vitro assay, 14% of wild-type activity
K87M
lyase activity is about 70% compared to wild-type enzyme
M118A
lyase activity is about 25% compared to wild-type enzyme
N83A
lyase activity is about 35% compared to wild-type enzyme
Q55A
lyase activity is about 30% compared to wild-type enzyme
R141I
lyase activity is about 15% compared to wild-type enzyme
R66A
in vitro assay, 15% of wild-type activity
R68A
lyase activity is about 10% compared to wild-type enzyme
R97A
in vitro assay, 37% of wild-type activity. Contrary to wild-type,a great deal of alpha helix is involved in circular dichroism
S150A
lyase activity is about 125% compared to wild-type enzyme
S161V
lyase activity is about 105% compared to wild-type enzyme
W13S
in vitro assay, 22% of wild-type activity. Contrary to wild-type,a great deal of alpha helix is involved in circular dichroism
W175S
in vitro assay, 14% of wild-type activity
Y59A
lyase activity is about 35% compared to wild-type enzyme
Y65F
lyase activity is less than 5% compared to wild-type enzyme
C116S
-
in vitro assay,11% of wild-type activity
-
E111Q
-
lyase activity is about 75% compared to wild-type enzyme
-
H33F
-
in vitro assay, 14% of wild-type activity
-
N83A
-
lyase activity is about 35% compared to wild-type enzyme
-
Q55A
-
lyase activity is about 30% compared to wild-type enzyme
-
R66A
-
in vitro assay, 15% of wild-type activity
-
R68A
-
lyase activity is about 10% compared to wild-type enzyme
-
R97A
-
in vitro assay, 37% of wild-type activity. Contrary to wild-type,a great deal of alpha helix is involved in circular dichroism
-
Y65F
-
lyase activity is less than 5% compared to wild-type enzyme
-
additional information