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4.4.1.3: dimethylpropiothetin dethiomethylase

This is an abbreviated version!
For detailed information about dimethylpropiothetin dethiomethylase, go to the full flat file.

Word Map on EC 4.4.1.3

Reaction

S,S-dimethyl-beta-propiothetin
=
Dimethyl sulfide
+
Acrylate

Synonyms

DddL lyase, dddP, DddQ, DddW, Dddy, desulfhydrase, dethiomethylase, dimethylpropiothetin, dimethylsulfoniopropionate lyase, DLA, DMSP, DMSP lyase, DMSP-lyase, F963_02809, SAMN05660830_03106, SL1157_2466

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.3 dimethylpropiothetin dethiomethylase

Engineering

Engineering on EC 4.4.1.3 - dimethylpropiothetin dethiomethylase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
environmental protection
D295A
-
complete loss of activity
D297A
-
complete loss of activity
D307A
-
complete loss of activity
E406A
-
complete loss of activity
E421A
-
complete loss of activity
H371A
-
complete loss of activity
D377A
D0CY07
complete loss of activity
D377N
D0CY07
about 10% residual activity
Y366A
D0CY07
20% residual activity
D377A
-
complete loss of activity
-
D377N
-
about 10% residual activity
-
Y366A
-
20% residual activity
-
additional information
-
Sulfitobacter J443, DddL-mutant has lost its Ddd+ phenotype, showing that this species has no other gene that can substitute for dddL, the wild type produces 385 nmol dimethylsulfide, the mutant produces only 0.7 nmol dimethylsulfide