4.4.1.23: 2-hydroxypropyl-CoM lyase

This is an abbreviated version!
For detailed information about 2-hydroxypropyl-CoM lyase, go to the full flat file.

Word Map on EC 4.4.1.23

4.4.1.23

vinyl

ethene

epoxide

alkene

groundwater

monooxygenase

nocardioides

mycobacterium

2-mercaptoethanesulfonic

bioremediation

epoxyethane

chlorinated

xanthobacter

rhodococcus

zn-deficient

propene

uv-visible

alkyltransferase

smegmatis

autotrophicus

pulsed-field

biocatalysis

microcosms

2-bromoethanesulfonate

thioether

degradation

Reaction

Synonyms

coenzyme M-epoxyalkane ligase, Ea-CoMT, EaCoMT, EC 4.2.99.19, epoxyalkane coenzyme M transferase, epoxyalkane:2-mercaptoethanesulfonate transferase, epoxyalkane:CoM transferase, epoxyalkyl:CoM transferase, epoxypropane:coenzyme M transferase, epoxypropyl:CoM transferase, EtnE, transferase, epoxyalkyl:coenzyme M

ECTree

4 Lyases

4.4 Carbon-sulfur lyases

4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)

4.4.1.23 2-hydroxypropyl-CoM lyase

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Results	in table
321	AA Sequence
1	Application
1	CAS Registry Number
3	Cloned(Commentary)
1	Inhibitors
2	KM Value [mM]
3	Metals/Ions
1	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
22	Organism
4	Pathway
2	pH Optimum
1	pH Range
4	Protein Variants
2	Purification (Commentary)
2	Reaction
1	Reaction Type
17	Reference
14	Specific Activity [micromol/min/mg]
34	Substrates and Products (Substrate)
1	Subunits
20	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Turnover Number [1/s]

Engineering

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Engineering on EC 4.4.1.23 - 2-hydroxypropyl-CoM lyase

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R257L

Mycobacterium sp. JS623

B9VTP0, B9VTP5

higher enzyme activities compared to wild-type, growth on vinyl chloride more rapidly than the wild-type strain

701828

W243G

Mycobacterium sp. JS623

B9VTP0, B9VTP5

higher enzyme activities compared to wild-type, growth on vinyl chloride more rapidly than the wild-type strain

701828

C220A

Xanthobacter autotrophicus

largely catalytically inactive protein, 0.06% of wild-type activity

393831

additional information

2 entries