4.4.1.22: S-(hydroxymethyl)glutathione synthase

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For detailed information about S-(hydroxymethyl)glutathione synthase, go to the full flat file.

Reaction

Synonyms

EC 1.2.1.1, Gfa, glutathione-dependent formaldehyde-activating enzyme

ECTree

     4 Lyases

         4.4 Carbon-sulfur lyases

             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)

                4.4.1.22 S-(hydroxymethyl)glutathione synthase

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Results	in table
2238	AA Sequence
1	CAS Registry Number
1	Cloned(Commentary)
2	Crystallization (Commentary)
3	General Information
2	Metals/Ions
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
3	Organism
6	Pathway
3	Purification (Commentary)
3	Reaction
1	Reaction Type
3	Reference
1	Specific Activity [micromol/min/mg]
5	Substrates and Products (Substrate)
1	Subunits
7	Synonyms
1	Systematic Name

Purification

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Purification on EC 4.4.1.22 - S-(hydroxymethyl)glutathione synthase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Paracoccus denitrificans

-

656068, 665643

recombinant His10-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration

Paracoccus denitrificans

Q51669

749071