4.4.1.22: S-(hydroxymethyl)glutathione synthase

This is an abbreviated version!
For detailed information about S-(hydroxymethyl)glutathione synthase, go to the full flat file.

Reaction

Synonyms

EC 1.2.1.1, Gfa, glutathione-dependent formaldehyde-activating enzyme

ECTree

     4 Lyases

         4.4 Carbon-sulfur lyases

             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)

                4.4.1.22 S-(hydroxymethyl)glutathione synthase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
2238	AA Sequence
1	CAS Registry Number
1	Cloned(Commentary)
2	Crystallization (Commentary)
3	General Information
2	Metals/Ions
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
3	Organism
6	Pathway
3	Purification (Commentary)
3	Reaction
1	Reaction Type
3	Reference
1	Specific Activity [micromol/min/mg]
5	Substrates and Products (Substrate)
1	Subunits
7	Synonyms
1	Systematic Name

Metals Ions

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Metals Ions on EC 4.4.1.22 - S-(hydroxymethyl)glutathione synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Metals and Ions Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Zn

Paracoccus denitrificans

-

the enzyme has a new fold with two zinc-sulfur centers, one that is structural (zinc tetracoordinated) and one catalytic (zinc apparently tricoordinated). In the complex of enzyme with glutathione, the catalytic zinc is displaced due to disulfide bond formation of glutathione with one of the zinc-coordinating cysteines

665643

Zn2+

Paracoccus denitrificans

Q51669

required, the GFA domain contains two zinc binding sites, one zinc ion is coordinated by four cysteinyl thiols (C31, C33, C99 and C102) in a tetrahedral geometry, whereas the other zinc ion is coordinated by three cysteinyl thiols (C52, C54 and C57) in a trigonal planar geometry. GSH binding induces translocation of the second zinc ion. Manual model building of the catalytic zinc site

749071