4.4.1.21: S-ribosylhomocysteine lyase

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For detailed information about S-ribosylhomocysteine lyase, go to the full flat file.

Reaction

Synonyms

AI-2 synthase, autoinducer-2 synthase, BsLuxS, EC 3.13.1.2, EC 3.2.1.148, EC 3.3.1.3, EcLuxS, lsrR, Lux S, LuxS, LuxS protein, S-ribosyl homocysteinase, S-ribosylhomocysteinase, S-ribosylhomocysteine lyase, S-ribosylhomocysteinelyase, VhLuxS

ECTree

     4 Lyases

         4.4 Carbon-sulfur lyases

             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)

                4.4.1.21 S-ribosylhomocysteine lyase

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Results	in table
2	Activating Compound
10500	AA Sequence
2	Application
1	CAS Registry Number
21	Cloned(Commentary)
7	Crystallization (Commentary)
1	Expression
34	General Information
2	General Stability
35	Inhibitors
5	kcat/KM [mM/s]
32	Ki Value [mM]
32	KM Value [mM]
35	Metals/Ions
1	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
183	Organism
1	Oxidation Stability
8	Pathway
11	PDB ID
1	pH Optimum
2	pI Value
46	Protein Variants
9	Purification (Commentary)
3	Reaction
13	Reaction Type
160	Reference
2	Source Tissue
1	Storage Stability
135	Substrates and Products (Substrate)
7	Subunits
188	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Stability [°C]
23	Turnover Number [1/s]

Application

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Application on EC 4.4.1.21 - S-ribosylhomocysteine lyase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

analysis

Escherichia coli

-

rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry assay for the simultaneous quantification of the metabolites and precursors of the activated methyl cycle. Analytes are extracted from Escherichia coli MG1655 and chemically derivatized as N(O,S)-iso-butyloxycarbonyl iso-butyl esters using iso-butyl chloroformate in an aqueous iso-butanol/pyridine environment. S-Adenosylmethionine, S-adenosylhomocysteine, S-ribosylhomocysteine, homocysteine, methionine, cystathionine, cysteine, and homoserine are quantified by liquid chromatography-positive ion tandem electrospray ionization mass spectrometry. Internal standards are isotopically labeled [13CD3]methionine and S-adenosylcysteine. Linearity of the assay is established up to a concentration of 700 microg/g cell dry weight for each analyte

713738

medicine

Escherichia coli

Q8X902

autoinducer-2 promotes interspecies signaling, the autoinducer-3 activates enterohemorrhagic Escherichia coli virulence genes, knocking out luxS in the enterohemorrhagic human pathogen Escherichia coli reveals a defect in AI-3 production, but not in AI-2 production

680464