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4.4.1.16: selenocysteine lyase

This is an abbreviated version!
For detailed information about selenocysteine lyase, go to the full flat file.

Word Map on EC 4.4.1.16

Reaction

L-selenocysteine
+
reduced acceptor
=
selenide
+
L-alanine
+
acceptor

Synonyms

ABA3-NifS, cpSL, CsdB, L-selenocysteine selenide-lyase, More, SCL, Scly, Sec lyase, selenocysteine beta-lyase, selenocysteine lyase, selenocysteine reductase, SLC, SufS

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.16 selenocysteine lyase

Cloned

Cloned on EC 4.4.1.16 - selenocysteine lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Brassica juncea using Agrobacterium tumefaciens to introduce genetic sequence, transformation results in enhanced enzyme activity but decreased Se tolerance
-
expressed in chloroplasts and cytosol of Arabidopsis sp., enhanced Se tolerance when enzyme is expressed in cytosol, decreased Se tolerance when expressed in chloroplasts
-
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli JM109
-
expression in Escherichia coli
expression in Escherichia coli into the pGEM-T Easy vector for sequencing, 5’ constructs with deletions in the promoter region are subcloned into the promoterless luciferase expression vector
-
expression in Escherichia coli rosetta
recombinant expression in Escherichia coli strain BL21(DE3)
-
recombinant expression of GFP-tagged enzyme in spermatids of mature testes of transgenic mice
-
recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
sequence comparisons, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
transfection of HepG2, Hepa12-6, Ht22 cells