4.3.2.5: peptidylamidoglycolate lyase

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For detailed information about peptidylamidoglycolate lyase, go to the full flat file.

Word Map on EC 4.3.2.5

4.3.2.5

glycine-extended

monooxygenase

alpha-hydroxylating

ascorbate

neuropeptides

1.14.17.3

entails

alpha-monooxygenase

alpha-ae

peptidyl-alpha-hydroxyglycine

4-phenyl-3-butenoic

dealkylation

prohormones

pro-peptide

ascorbate-dependent

miller

Reaction

Synonyms

alpha-AE, alpha-hydroxyglycine amidating dealkylase, bifunctional peptidylglycine alpha-amidating enzyme, dPAL1 gene product, dPAL2 gene product, HGAD, lyase, peptidyl-alpha hydroxyglycine, PAL, PAL2, peptidyl-alpha-hydroxyglycine alpha-amidating lyase, peptidylamidoglycolate lyase, PGL, rPAL gene product

ECTree

4 Lyases

4.3 Carbon-nitrogen lyases

4.3.2 Amidine-lyases

4.3.2.5 peptidylamidoglycolate lyase

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Results	in table
4	Activating Compound
1091	AA Sequence
1	CAS Registry Number
10	Cloned(Commentary)
2	Cofactor
2	General Information
23	Inhibitors
9	Ki Value [mM]
30	KM Value [mM]
12	Localization
13	Metals/Ions
10	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
89	Organism
13	PDB ID
6	pH Optimum
12	Protein Variants
10	Purification (Commentary)
1	Reaction
1	Reaction Type
29	Reference
25	Source Tissue
11	Specific Activity [micromol/min/mg]
1	Storage Stability
36	Substrates and Products (Substrate)
3	Subunits
27	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
6	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 4.3.2.5 - peptidylamidoglycolate lyase

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0.25 - 0.32

Rattus norvegicus

dansyl-Tyr-Val-Gly as substrate

438571

0.53

Homo sapiens

Sephacryl S-300 purification step

12000

Superose 12 pool

16000000

Mono Q fraction

180000

monofunctional lyase: PGL

648705

23000

Xenopus laevis

PGL domain of full expressed enzyme: AE

648705

4.5 - 8.3

Rattus norvegicus

dansyl-Tyr-Val-alpha-hydroxyglycine as substrate

438571

510

Bos taurus

Mono Q pool A, after concentration

648691

57.24

Rattus norvegicus

affinity chromatography step II

648700

76000

Xenopus laevis

PGL domain of transmembrane truncated enzyme: dAE

648705

additional information

Homo sapiens

P19021

691012