4.3.2.5: peptidylamidoglycolate lyase

This is an abbreviated version!
For detailed information about peptidylamidoglycolate lyase, go to the full flat file.

Reaction

Synonyms

alpha-AE, alpha-hydroxyglycine amidating dealkylase, bifunctional peptidylglycine alpha-amidating enzyme, dPAL1 gene product, dPAL2 gene product, HGAD, lyase, peptidyl-alpha hydroxyglycine, PAL, PAL2, peptidyl-alpha-hydroxyglycine alpha-amidating lyase, peptidylamidoglycolate lyase, PGL, rPAL gene product

ECTree

     4 Lyases

         4.3 Carbon-nitrogen lyases

             4.3.2 Amidine-lyases

                4.3.2.5 peptidylamidoglycolate lyase

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Results	in table
4	Activating Compound
1091	AA Sequence
1	CAS Registry Number
10	Cloned(Commentary)
2	Cofactor
2	General Information
23	Inhibitors
9	Ki Value [mM]
30	KM Value [mM]
12	Localization
13	Metals/Ions
10	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
89	Organism
13	PDB ID
6	pH Optimum
12	Protein Variants
10	Purification (Commentary)
1	Reaction
1	Reaction Type
29	Reference
25	Source Tissue
11	Specific Activity [micromol/min/mg]
1	Storage Stability
36	Substrates and Products (Substrate)
3	Subunits
27	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
6	Turnover Number [1/s]

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Reference on EC 4.3.2.5 - peptidylamidoglycolate lyase

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

438567

Perkins, S.N.; Husten, E.J.; Eipper, B.A.

The 108-kDA peptidylglycine alpha-amidating monooxygenase precursor contains two separable enzymatic activities involved in peptide amidation

Biochem. Biophys. Res. Commun.

171

926-932

1990

Bos taurus

2222453

brenda

438569

Suzuki, K.; Shimoi, H.; Iwasaki, Y.; Kawahara, T.; Matsuura, Y.; Nishikawa, Y.

Elucidation of amidating reaction mechanism by frog amidating enzyme, peptidylglycine alpha-hydroxylating monooxygenase, expressed in insect cell culture

EMBO J.

9

4259-4265

1990

Xenopus laevis

2265607

brenda

438571

Merkler, D.J.; Young, S.D.

Recombinant type A rat 75-kDa alpha-amidating enzyme catalyzes the conversion of glycine-extended peptides to peptide amides via an alpha-hydroxyglycine intermediate

Arch. Biochem. Biophys.

289

192-196

1991

Rattus norvegicus

1898062

brenda

438592

Ping, D.; Mounier, C.E.; May, S.W.

Reaction versus subsite stereospecificity of peptidylglycine alpha-monooxygenase and peptidylamidoglycolate lyase, the two enzymes involved in peptide amidation

J. Biol. Chem.

270

29250-29255

1995

Bos taurus

7493955

brenda

438594

Francisco, W.A.; Merkler, D.J.; Blackburn, N.J.; Klinman, J.P.

Kinetic mechanism and intrinsic isotope effects for the peptidylglycine alpha-amidating enzyme reaction

Biochemistry

37

8244-8252

1998

Rattus norvegicus

9609721

brenda

648691

Katopodis, A.G.; Ping, D.; May, S.W.

A novel enzyme from bovine neurointermediate pituitary catalyzes dealkylation of alpha-hydroxyglycine derivatives, thereby functioning sequentially with peptidylglycine alpha-amidating monooxygenase in peptideamidation

Biochemistry

29

6115-6120

1990

Bos taurus

2207061

brenda

648692

Katopodis, A.G.; Ping, D.; Smith, C.E.; May, S.W.

Functional and structural characterization of peptidylamidoglycolate lyase, the enzyme catalyzing the second step in peptide amidation

Biochemistry

30

6189-6194

1991

Bos taurus

2059626

brenda

648693

Husten, E.J.; Tausk, F.A.; Keutmann, H.T.; Eipper, B.A.

Use of endoproteases to identify catalytic domains, linker regions, and functional interactions in soluble peptidylglycine alpha-amidating monooxygenase

J. Biol. Chem.

268

9709-9717

1993

Bos taurus, Rattus norvegicus

8486658

brenda

648694

Ping, D.; Katopodis, A.G.; May, S.W.

Tandem stereochemistry of peptidylglycine alpha-monooxygenase and peptidylamidoglycolate lyase, the two enzymes involved in peptide amidation

J. Am. Chem. Soc.

114

3998-4000

1992

Bos taurus

-

brenda

648695

Husten, E.J.; Eipper, B.A.

The membrane-bound bifunctional peptidylglycine alpha-amidating monooxygenase protein. Exploration of its domain structure through limited proteolysis.

J. Biol. Chem.

266

17004-17010

1991

Rattus norvegicus

1894599

brenda

648697

Eipper, B.A.; Perkins, S.N.; Husten, E.J.; Johnson, R.C.; Keutmann, H.T.; Mains, R.E.

Peptidyl-alpha-hydroxyglycine alpha-amidating lyase. Purification, characterization, and expressio

J. Biol. Chem.

266

7827-7833

1991

Bos taurus

1902227

brenda

648700

Takahashi, K.; Okamoto, H.; Seino, H.; Noguchi, M.

Peptidylglycine alpha-amidating reaction: evidence for a two-step mechanism involving a stable intermediate at neutral pH

Biochem. Biophys. Res. Commun.

169

524-530

1990

Bos taurus, Rattus norvegicus

2357219

brenda

648701

Kato, I.; Yonekura, H.; Tajima, M.; Yanagi, M.; Yamamoto, H.; Okamoto, H.

Two enzymes concerned in peptide hormone alpha-amidation are synthesized from a single mRN

Biochem. Biophys. Res. Commun.

172

197-203

1990

Rattus norvegicus

1699535

brenda

648703

Satani, M.; Takahashi, K.; Sakamoto, H.; Harada, S.; Kaida, Y.; Noguchi, M.

Expression and characterization of human bifunctional peptidylglycine alpha-amidating monooxygenase

Protein Expr. Purif.

28

293-302

2003

Homo sapiens

12699694

brenda

648704

Kolhekar, A.S.; Bell, J.; Shiozaki, E.N.; Jin, L.; Keutmann, H.T.; Hand, T.A.; Mains, R.E.; Eipper, B.A.

Essential features of the catalytic core of peptidyl-a-hydroxyglycine alpha-amidating Lyase

Biochemistry

41

12384-12394

2002

Rattus norvegicus

12369828

brenda

648705

Moore, A.B.; May, S.W.

Kinetic and inhibition studies on substrate channelling in the bifunctional enzyme catalysing C-terminal amidation

Biochem. J.

341

33-40

1999

Bos taurus, Xenopus laevis

10377242

brenda

648706

Garmendia, O.; Rodriguez, M.P.; Burrell, M.A.; Villaro, A.C.

Immunocytochemical finding of the amidating enzymes in mouse pancreatic A-, B-, and D-cells: a comparison with human and rat

J. Histochem. Cytochem.

50

1401-1415

2002

Homo sapiens, Mus musculus, Rattus norvegicus

12364573

brenda

664714

Park, D.; Han, M.; Kim, Y.C.; Han, K.A.; Taghert, P.H.

Ap-let neurons-a peptidergic circuit potentially controlling ecdysial behavior in Drosophila

Dev. Biol.

269

95-108

2004

Drosophila melanogaster (Q9W1L5)

15081360

brenda

666112

Han, M.; Park, D.; Vanderzalm, P.J.; Mains, R.E.; Eipper, B.A.; Taghert, P.H.

Drosophila uses two distinct neuropeptide amidating enzymes, dPAL1 and dPAL2

J. Neurochem.

90

129-141

2004

Rattus norvegicus (P14925), Drosophila melanogaster (Q9V5E1), Drosophila melanogaster (Q9W1L5)

15198673

brenda

691012

Takahashi, K.; Harada, S.; Higashimoto, Y.; Shimokawa, C.; Sato, H.; Sugishima, M.; Kaida, Y.; Noguchi, M.

Involvement of metals in enzymatic and nonenzymatic decomposition of C-terminal alpha-hydroxyglycine to amide: An implication for the catalytic role of enzyme-bound zinc in the peptidylamidoglycolate lyase reaction

Biochemistry

48

1654-1662

2009

Homo sapiens (P19021)

19170548

brenda

730684

Yin, P.; Bousquet-Moore, D.; Annangudi, S.P.; Southey, B.R.; Mains, R.E.; Eipper, B.A.; Sweedler, J.V.

Probing the production of amidated peptides following genetic and dietary copper manipulations

PLoS ONE

6

e28679

2011

Mus musculus, Mus musculus C57/BL6J

22194882

brenda

752742

Bell, J.; Ash, D.E.; Snyder, L.M.; Kulathila, R.; Blackburn, N.J.; Merkler, D.J.

Structural and functional investigations on the role of zinc in bifunctional rat peptidylglycine alpha-amidating enzyme

Biochemistry

36

16239-16246

1997

Rattus norvegicus (P14925)

9405058

brenda

Entries 1 - 20 of 22

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