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4.3.2.5: peptidylamidoglycolate lyase

This is an abbreviated version!
For detailed information about peptidylamidoglycolate lyase, go to the full flat file.

Word Map on EC 4.3.2.5

Reaction

[peptide]-(2S)-2-hydroxyglycine
=
[peptide]-amide
 +
glyoxylate

Synonyms

alpha-AE, alpha-hydroxyglycine amidating dealkylase, bifunctional peptidylglycine alpha-amidating enzyme, dPAL1 gene product, dPAL2 gene product, HGAD, lyase, peptidyl-alpha hydroxyglycine, PAL, PAL2, peptidyl-alpha-hydroxyglycine alpha-amidating lyase, peptidylamidoglycolate lyase, PGL, rPAL gene product

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.2 Amidine-lyases
                4.3.2.5 peptidylamidoglycolate lyase

KM Value

KM Value on EC 4.3.2.5 - peptidylamidoglycolate lyase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
(R)-2-phenylbutyl-alpha-hydroxy-Gly
-
37°C, pH 6.5
2.6
(R)-O-acetyl-mandelyl-alpha-hydroxyglycine
-
37°C, pH 6.5
0.33
(S)-2-phenylbutyl-alpha-hydroxy-Gly
-
37°C, pH 6.5
0.56
(S)-O-acetyl-mandelyl-alpha-hydroxyglycine
-
37°C, pH 6.5
0.5
acetyl-D-Phe-alpha-hydroxyglycine
-
37°C, pH 6.5
0.23
acetyl-L-Phe-alpha-hydroxyglycine
-
37°C, pH 6.5
0.013 - 0.238
alpha-N-Acetyl-Tyr-Val-alpha-hydroxyglycine
0.035 - 0.048
Dansyl-Tyr-Val-alpha-hydroxyglycine
-
37°C, pH 7.0
0.0023 - 0.004
dansyl-Tyr-Val-Gly
-
37°C, pH 6
0.0215 - 0.0887
N-2,4,6-trinitrophenyl-D-Tyr-L-Val-(S)-alpha-hydroxy-Gly
0.46
N-benzoyl-alpha-hydroxy-Gly
-
37°C, pH 6.5
0.0102 - 0.33
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine
0.014 - 0.033
trinitrophenyl-D-Tyr-L-Val-alpha-hydroxy-Gly
additional information
additional information
apparent second-order rate constants at different pHs
-