4.3.2.2: adenylosuccinate lyase
This is an abbreviated version!
For detailed information about adenylosuccinate lyase, go to the full flat file.
Word Map on EC 4.3.2.2
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4.3.2.2
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purine
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saicar
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succinyladenosine
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autistic
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carboxamide
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psychomotor
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imp
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succinylaminoimidazole
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amylosucrase
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ribotide
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geothermalis
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aminoimidazole
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drug development
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medicine
- 4.3.2.2
- purine
-
saicar
-
succinyladenosine
-
autistic
- carboxamide
-
psychomotor
- imp
-
succinylaminoimidazole
- amylosucrase
- ribotide
- geothermalis
-
aminoimidazole
- drug development
- medicine
Reaction
Synonyms
adenylosuccinase, adenylosuccinate lyase, ADL, ADSL, AMPS lyase, ASASE, ASL, Glutamyl-tRNA synthetase regulatory factor, lyase, adenylosuccinate, PurB, succino AMP-lyase, succino-AMP lyase
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Purification
Purification on EC 4.3.2.2 - adenylosuccinate lyase
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recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta 2(DE3)pLysS by nickel affinity chromatography
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recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta 2(DE3)pLysS by nickel affinity chromatography, dialysis, cleavage of the His-tag through thrombin, and gel filtration, to homogeneity
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Bl21 by nickel affinity chromatography
using a Ni-NTA column, the 6-histidine tag is removed by cleavage with thrombin, separation of thrombin and histidine-tag from the enzyme is achieved by using a Sephadex G-100 column
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