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4.3.2.2: adenylosuccinate lyase

This is an abbreviated version!
For detailed information about adenylosuccinate lyase, go to the full flat file.

Word Map on EC 4.3.2.2

Reaction

(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
=
fumarate
+
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

Synonyms

adenylosuccinase, adenylosuccinate lyase, ADL, ADSL, AMPS lyase, ASASE, ASL, Glutamyl-tRNA synthetase regulatory factor, lyase, adenylosuccinate, PurB, succino AMP-lyase, succino-AMP lyase

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.2 Amidine-lyases
                4.3.2.2 adenylosuccinate lyase

Molecular Weight

Molecular Weight on EC 4.3.2.2 - adenylosuccinate lyase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
147000
-
determined by native polyacrylamide gel electrophoresis, wild-type enzyme
148000
-
determined by native polyacrylamide gel electrophoresis, mutant R310K
152000
-
mutant N270L, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride
153000
-
wild type, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride
155000
-
mutant D69E, light scattering method, 0.25 mg/ml
159000
166000
-
mutant N270D, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride
173000
174000
-
mutant Q212M, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride
176000
181000
-
light scattering method, 0.25 mg/ml
182000
-
determined by light scattering, mutant R310K
191000
192000
-
mutant S263H, light scattering
193000
-
determined by native polyacrylamide gel electrophoresis, mutant S93A
194000
194800
-
determined by analytical ultracentrifugation, mutant T140A
196000
197000
-
mutant S263A, light scattering
199000
-
mutant S262H, light scattering
199700
-
determined by analytical ultracentrifugation, wild-type enzyme
200000
201000
-
mutant enzyme S413P, gel filtration
209000
-
mutant I62E/D65R, native PAGE
210000
-
native PAGE
214500
recombinant His-tagged R303C mutant enzyme
216400
-
determined by analytical ultracentrifugation, mutant S306A
217000
-
mutant I62D/D65R, native PAGE
219000
-
mutant R301Q, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride
221000
mutant S290H, light scattering
222000
-
analytical ultracentrifugation
223000
gel filtration, at 22°C
225000
recombinant His-tagged wild-type enzyme
227000
228000
-
gel filtration, His-tagged protein
230000
analytical ultracentrifugation, at 25°C
234000
237000
mutant S289A, light scattering
300000
-
gel filtration
49490
-
x * 49490, calculation from nucleotide sequence
49600
4 * 49600, calculated. Enzyme forms a dimer of dimers, crystallization data
50000
52000
54552
x * 54552, calculated
55000
56000
56235
4 * 55000, recombinant His-tagged enzyme, SDS-PAGE, 4 * 56235, recombinant His-tagged enzyme, mass spectrometry
57000