4.3.2.2: adenylosuccinate lyase

This is an abbreviated version!
For detailed information about adenylosuccinate lyase, go to the full flat file.

Reaction

Synonyms

adenylosuccinase, adenylosuccinate lyase, ADL, ADSL, AMPS lyase, ASASE, ASL, Glutamyl-tRNA synthetase regulatory factor, lyase, adenylosuccinate, PurB, succino AMP-lyase, succino-AMP lyase

ECTree

     4 Lyases

         4.3 Carbon-nitrogen lyases

             4.3.2 Amidine-lyases

                4.3.2.2 adenylosuccinate lyase

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Results	in table
44124	AA Sequence
8	Application
1	CAS Registry Number
22	Cloned(Commentary)
13	Crystallization (Commentary)
462	Disease/ Diagnostics
1	Expression
24	General Information
6	General Stability
74	Inhibitors
9	kcat/KM [mM/s]
3	Ki Value [mM]
72	KM Value [mM]
2	Localization
3	Metals/Ions
72	Molecular Weight [Da]
88	Natural Substrates/ Products (Substrates)
69	Organism
11	Pathway
82	PDB ID
21	pH Optimum
3	pH Range
102	Protein Variants
19	Purification (Commentary)
9	Reaction
10	Reaction Type
69	Reference
29	Source Tissue
69	Specific Activity [micromol/min/mg]
7	Storage Stability
145	Substrates and Products (Substrate)
34	Subunits
52	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
5	Temperature Stability [°C]
12	Turnover Number [1/s]

KM Value

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KM Value on EC 4.3.2.2 - adenylosuccinate lyase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0111

(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate

Leishmania braziliensis

A4H3W5

pH 7.5, 25°C

749293

0.0437

2',3'-dideoxyadenylosuccinate

Saccharomyces cerevisiae

-

-

34464

0.0008 - 0.003

2'-deoxy-succino-AMP

2 entries

0.001 - 0.0081

4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide

2 entries

0.0003 - 0.0029

5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide

3 entries

0.0095

8-aza-succino-AMP

Leishmania donovani

-

-

1541

0.00146 - 0.023

adenylosuccinate

13 entries

0.0013 - 0.0551

adenylsuccinate

13 entries

0.004 - 0.048

AMP

4 entries

0.0027 - 0.0056

arabinosyl-succino-AMP

2 entries

0.035 - 0.76

fumarate

3 entries

0.02

mercaptopurinosuccinate

Rattus norvegicus

-

-

34456

0.0012 - 0.013

N6-(1,2-dicarboxyethyl)AMP

9 entries

0.0007 - 0.009

phosphoribosylsuccinyl-aminoimidazole carboxamide

3 entries

0.011

succino-4-aminopyrazolo[3,4-d]pyrimidine ribose 5'-phosphate

Leishmania donovani

-

-

1541

0.0014 - 0.2042

succinyladenosine monophosphate

7 entries

additional information

additional information

8 entries

-

0.0008

2'-deoxy-succino-AMP

Leishmania donovani

-

-

1541

0.003

2'-deoxy-succino-AMP

Mus musculus

-

-

34457

0.001

4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide

Rattus norvegicus

-

-

34449

0.0081

4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide

Rattus norvegicus

-

-

34456

0.0003

5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide

Homo sapiens

P30566

pH 7.0, 25°C, mutant S290A

695003

0.0016

5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide

Homo sapiens

P30566

pH 7.0, 25°C, wild-type enzyme

695003

0.0029

5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide

Bacillus subtilis

-

pH 7.0, 25°C, wild-type enzyme

695003

0.00146

adenylosuccinate

Homo sapiens

-

histidine-tagged enzyme

682657

0.0015

adenylosuccinate

Bacillus subtilis

-

mutant D69E

682733

0.00177

adenylosuccinate

Bacillus subtilis

-

wild-type enzyme

682738

0.00178

adenylosuccinate

Homo sapiens

-

none-tagged enzyme

682657

0.0021

adenylosuccinate

Bacillus subtilis

-

wild-type enzyme

682733

0.00224

adenylosuccinate

Bacillus subtilis

-

mutant S94A

682738

0.0027

adenylosuccinate

Bacillus subtilis

-

wild type, 50 mM HEPES, pH 7.0, 25°C

664041

0.0071

adenylosuccinate

Bacillus subtilis

-

mutant R310K

682733

0.0093

adenylosuccinate

Bacillus subtilis

-

mutant D69N

682733

0.0128

adenylosuccinate

Bacillus subtilis

-

mutant S306A

682738

0.0177

adenylosuccinate

Bacillus subtilis

-

mutant T93A

682738

0.0192

adenylosuccinate

Bacillus subtilis

-

mutant T140A

682738

0.023

adenylosuccinate

Bacillus subtilis

-

mutant R310Q

682733

0.0013

adenylsuccinate

Bacillus subtilis

-

mutant D69E, 50 mM HEPES, pH 7.0, 25°C

665595

0.002

adenylsuccinate

Bacillus subtilis

-

mutant D65R/D69E, 50 mM HEPES, pH 7.0, 25°C

665595

0.004

adenylsuccinate

Homo sapiens

-

50 mM HEPES, pH 7.0, 25°C

665595

0.0043

adenylsuccinate

Bacillus subtilis

-

mutant I62E/D69E, 50 mM HEPES, pH 7.0, 25°C

665595

0.0046

adenylsuccinate

Bacillus subtilis

-

mutant D65R, 50 mM HEPES, pH 7.0, 25°C

665595

0.0061

adenylsuccinate

Bacillus subtilis

-

mutant I62E/D65R/D69E, 50 mM HEPES, pH 7.0, 25°C

665595

0.0107

adenylsuccinate

Bacillus subtilis

-

mutant I62D/D65R/D69E, 50 mM HEPES, pH 7.0, 25°C

665595

0.0166

adenylsuccinate

Bacillus subtilis

-

mutant Q212E, 50 mM HEPES, pH 7.0, 25°C

664041

0.0175

adenylsuccinate

Bacillus subtilis

-

mutant I62E, 50 mM HEPES, pH 7.0, 25°C

665595

0.0177

adenylsuccinate

Bacillus subtilis

-

mutant I62D/D69E, 50 mM HEPES, pH 7.0, 25°C

665595

0.0181

adenylsuccinate

Bacillus subtilis

-

mutant I62E/D65R, 50 mM HEPES, pH 7.0, 25°C

665595

0.0488

adenylsuccinate

Bacillus subtilis

-

mutant I62D/D65R, 50 mM HEPES, pH 7.0, 25°C

665595

0.0551

adenylsuccinate

Bacillus subtilis

-

mutant I62D, 50 mM HEPES, pH 7.0, 25°C

665595

0.004

AMP

Leishmania donovani

-

-

1541

0.034

AMP

Plasmodium falciparum

O15918

pH 7.4, 25°C

691116

0.036

AMP

Artemia sp.

-

-

34451

0.048

AMP

Saccharomyces cerevisiae

-

-

34458

0.0027

arabinosyl-succino-AMP

Leishmania donovani

-

-

1541

0.0056

arabinosyl-succino-AMP

Mus musculus

-

-

34457

0.035

fumarate

Artemia sp.

-

-

34451

0.52

fumarate

Saccharomyces cerevisiae

-

-

34458

0.76

fumarate

Plasmodium falciparum

O15918

pH 7.4, 25°C

691116

0.0012

N6-(1,2-dicarboxyethyl)AMP

Artemia sp.

-

-

34451

0.0015

N6-(1,2-dicarboxyethyl)AMP

Rattus norvegicus

-

-

34449

0.00254

N6-(1,2-dicarboxyethyl)AMP

Homo sapiens

-

enzyme from cell line 1751

34462

0.00289

N6-(1,2-dicarboxyethyl)AMP

Homo sapiens

-

enzyme from cell line 1753

34462

0.0033

N6-(1,2-dicarboxyethyl)AMP

Leishmania donovani

-

-

1541

0.0033

N6-(1,2-dicarboxyethyl)AMP

Homo sapiens

-

enzyme from wild-type cell lines

34462

0.0047

N6-(1,2-dicarboxyethyl)AMP

Mus musculus

-

-

34457

0.012

N6-(1,2-dicarboxyethyl)AMP

Saccharomyces cerevisiae

-

-

34458

0.013

N6-(1,2-dicarboxyethyl)AMP

Rattus norvegicus

-

-

34456

0.0007

phosphoribosylsuccinyl-aminoimidazole carboxamide

Homo sapiens

-

recombinant His-tagged mutant R426H, pH 7.4, 25°C

729296

0.00174

phosphoribosylsuccinyl-aminoimidazole carboxamide

Homo sapiens

-

recombinant His-tagged wild-type enzyme, pH 7.4, 25°C

729296

0.009

phosphoribosylsuccinyl-aminoimidazole carboxamide

Homo sapiens

-

recombinant His-tagged mutant R303C, pH 7.4, 25°C

729296

0.0014

succinyladenosine monophosphate

Homo sapiens

-

recombinant His-tagged mutant R426H, pH 7.4, 25°C

729296

0.0021

succinyladenosine monophosphate

Homo sapiens

-

recombinant His-tagged wild-type enzyme, pH 7.4, 25°C

729296

0.0027

succinyladenosine monophosphate

Homo sapiens

-

recombinant His-tagged mutant R303C, pH 7.4, 25°C

729296

0.0091

succinyladenosine monophosphate

Homo sapiens

-

wild-type enzyme, pH 8.1, 37°C

730526

0.032

succinyladenosine monophosphate

Plasmodium falciparum

O15918

pH 7.4, 25°C

691116

0.0437

succinyladenosine monophosphate

Mycolicibacterium smegmatis

A0R4I6

pH 7.6, 37°C

729717

0.2042

succinyladenosine monophosphate

Mycobacterium tuberculosis

-

pH 7.6, 37°C

729717

additional information

additional information

Homo sapiens

-

pH-dependence of Km-value

34461

-

additional information

additional information

Mycobacterium tuberculosis

-

Michaelis-Menten kinetics

729717

-

additional information

additional information

Mycolicibacterium smegmatis

A0R4I6

Michaelis-Menten kinetics

729717

-

additional information

additional information

Plasmodium falciparum

O15918

thermodynamics and kinetics, initial velocity and transient kinetics of succinyladenosine monophosphate cleavage, overview

691116

-

additional information

additional information

Plasmodium falciparum

-

thermodynamics and kinetics, initial velocity and transient kinetics of succinyladenosine monophosphate cleavage, overview

691116

-

additional information

additional information

Homo sapiens

-

non-linear dependence of the activities on the substrate ratios due to competitive binding. The enzyme does not follow simple Michaelis-Menten kinetics, kinetic analysis of wild-type and mutant enzymes, overview. Wild-type enzyme and mutant R426H appear to have equivalent cooperative binding on both substrates, while mutant R303C demonstrates no cooperativity

729296

-

additional information

additional information

Homo sapiens

P30566

the enzyme shows cooperativity and does not follow simple Michaelis-Menten kinetics, kinetic analysis, overview

729218

-

additional information

additional information

Homo sapiens

-

the enzyme shows cooperativity and does not follow simple Michaelis-Menten kinetics, kinetic analysis, overview

729218

-