4.3.2.2: adenylosuccinate lyase
This is an abbreviated version!
For detailed information about adenylosuccinate lyase, go to the full flat file.
Word Map on EC 4.3.2.2
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4.3.2.2
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purine
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saicar
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succinyladenosine
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autistic
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carboxamide
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psychomotor
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imp
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succinylaminoimidazole
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amylosucrase
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ribotide
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geothermalis
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aminoimidazole
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drug development
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medicine
- 4.3.2.2
- purine
-
saicar
-
succinyladenosine
-
autistic
- carboxamide
-
psychomotor
- imp
-
succinylaminoimidazole
- amylosucrase
- ribotide
- geothermalis
-
aminoimidazole
- drug development
- medicine
Reaction
Synonyms
adenylosuccinase, adenylosuccinate lyase, ADL, ADSL, AMPS lyase, ASASE, ASL, Glutamyl-tRNA synthetase regulatory factor, lyase, adenylosuccinate, PurB, succino AMP-lyase, succino-AMP lyase
ECTree
4.3.2.2 adenylosuccinate lyaseAdvanced search results
results (
results (Inhibitors
Inhibitors on EC 4.3.2.2 - adenylosuccinate lyase
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
5-aminoimidazole-4-carboxamide ribonucleoside
50% inhibition at 0.167 mM
5-aminoimidazole-4-carboxamide riboside 5'-monophosphate
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i.e. ZMP or AICAR, product inhibition, accumulation in the brain of Lesch-Nyhan disease patients induces inhibition of mitochondrial oxidative phosphorylation and adenylosuccinate lyase activity, followed by dowregulation of ATP levels and multifocal cell death in the cerebellum
ammonium salt of N6-malonyl adenosine 5'-phosphate
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noncompetitive inhibitor, irreversible
erythro-beta-fluoro-4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide
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KBr
presence of increasing concentrations of KBr of 0.12.5 M disrupt electrostatic interactions leading to ASL dissociation and loss of catalytic activity
KCl
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stimulates up to maximum concentration of 80 mM, inhibition at higher concentration
N-(5-amino-1-(beta-D-ribofuranosyl)imidazole-4-carbonyl)-L-threo-beta-methylaspartic acid 5'-phosphate
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N6-(L-1-carboxy-2-methylsulfoxyethyl)-AMP
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i.e. adenylomethylsulfoxypropionate
PO43-
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activates at low concentration, inhibits at high concentrations above 25 mM
SO42-
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activates at low concentrations, inhibits at concentrations above 25 mM
threo-beta-4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide
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adenosine phosphonobutyric acid, 2'(3'),5'-diphosphate
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i.e. APBADP, the non-cleavable substrate analogue acts as a competitive inhibitor with respect to either substrate. ASL binds up to 4 mol of APBADP per mole of enzyme tetramer, the enzyme exhibits negative cooperativity. Binding to enzyme mutants, overview
adenosine phosphonobutyric acid, 2'(3'),5'-diphosphate
i.e. APBADP, the non-cleavable substrate analogue acts as a competitive inhibitor with respect to either substrate. ASL binds up to 4 mol of APBADP per mole of enzyme tetramer, the enzyme exhibits positive cooperativity
AMP
product inhibition competitive versus succinyladenosine monophosphate
trans-4-hydroxy-2-nonenal
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10-15 µM, inhibitor reacts both with the free enzyme and the enzyme substrate complex
trans-4-hydroxy-2-nonenal
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10-15 µM, inhibitor reacts both with the free enzyme and the enzyme substrate complex
trans-4-hydroxy-2-nonenal
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10-15 µM, inhibitor reacts both with the free enzyme and the enzyme substrate complex
trans-4-hydroxy-2-nonenal
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10-15 µM, inhibitor reacts both with the free enzyme and the enzyme substrate complex
additional information
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dithiothreitol increases inhibition of trans-4-hydroxy-2-nonenal; hydroxylamine reverses inhibition of trans-4-hydroxy-2-nonenal
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additional information
as hydrophobic interactions are weakened at 8°C and 4°C, the catalytic activity of ASL decreases strikingly and the enzyme dissociates to a mixture of monomer-dimer-trimer, with small amounts of tetramer
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additional information
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as hydrophobic interactions are weakened at 8°C and 4°C, the catalytic activity of ASL decreases strikingly and the enzyme dissociates to a mixture of monomer-dimer-trimer, with small amounts of tetramer
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additional information
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dithiothreitol increases inhibition of trans-4-hydroxy-2-nonenal; hydroxylamine reverses inhibition of trans-4-hydroxy-2-nonenal
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additional information
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dithiothreitol increases inhibition of trans-4-hydroxy-2-nonenal; hydroxylamine reverses inhibition of trans-4-hydroxy-2-nonenal
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additional information
-
dithiothreitol increases inhibition of trans-4-hydroxy-2-nonenal; hydroxylamine reverses inhibition of trans-4-hydroxy-2-nonenal
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