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4.3.1.7: ethanolamine ammonia-lyase

This is an abbreviated version!
For detailed information about ethanolamine ammonia-lyase, go to the full flat file.

Word Map on EC 4.3.1.7

Reaction

ethanolamine
=
acetaldehyde
+
NH3

Synonyms

AEL, ammonia-lyase, ethanolamine, EAL, ethanolamine ammonia lyase, ethanolamine ammonia-lyase, ethanolamine ammonia-lyase BMC, ethanolamine deaminase, ethanolamine-ammonia lyase, eut-L, EutB, eutBC

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.1 Ammonia-lyases
                4.3.1.7 ethanolamine ammonia-lyase

Purification

Purification on EC 4.3.1.7 - ethanolamine ammonia-lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
31% yield after DEAE-cellulose column
-
by nickel-affinity chromatography
-
N-terminal truncation of the Escherichia coli EAL beta-subunit dramatically increases the solubility of the enzyme without altering its catalytic properties
using Ni-NTA chromatography
-