4.3.1.7: ethanolamine ammonia-lyase
This is an abbreviated version!
For detailed information about ethanolamine ammonia-lyase, go to the full flat file.
Word Map on EC 4.3.1.7
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4.3.1.7
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b12-dependent
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adenosylcobalamin
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adenosylcobalamin-dependent
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cobiialamin
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5'-deoxyadenosine
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homolysis
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cobamide
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corrin
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5\'-deoxyadenosyl
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adocbl
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adocbl-dependent
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cobalt-carbon
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aminoethanol
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x-band
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full-spectrum
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metabolosome
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2h-labeled
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carbon-cobalt
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cryotrapped
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cryosolvent
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2-aminoethanols
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corrinoids
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eseem
- 4.3.1.7
-
b12-dependent
- adenosylcobalamin
-
adenosylcobalamin-dependent
-
cobiialamin
- 5'-deoxyadenosine
-
homolysis
- cobamide
-
corrin
-
5\'-deoxyadenosyl
-
adocbl
-
adocbl-dependent
-
cobalt-carbon
- aminoethanol
-
x-band
-
full-spectrum
-
metabolosome
-
2h-labeled
-
carbon-cobalt
-
cryotrapped
-
cryosolvent
- 2-aminoethanols
- corrinoids
-
eseem
Reaction
Synonyms
AEL, ammonia-lyase, ethanolamine, EAL, ethanolamine ammonia lyase, ethanolamine ammonia-lyase, ethanolamine ammonia-lyase BMC, ethanolamine deaminase, ethanolamine-ammonia lyase, eut-L, EutB, eutBC
ECTree
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Cloned
Cloned on EC 4.3.1.7 - ethanolamine ammonia-lyase
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cloning and overexpression of three different versions (eut-LpET151 his-tagged version (appends a cleavable 33-amino acid tag sequence to the N-terminus), Eut-L_NHIS (short His6-tagged version) and Eut-L_CHIS (short His6-tagged version)) of the protein is carried out directly from the Escherichia coli genome by selective PCR amplification. Selenomethione-derivatized proteins are obtained by growing cloned bacteria in selenomethionine-containing M9 minimal media. Protein overexpression and purification are performed.
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cloning in Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence
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Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence
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expressed as a His-taggd fusion protein
expression in Escherichia coli
expression in Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequences
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N-terminal His6-tagged beta subunit lacking residues Lysbeta4-Cysbeta43
overexpression in Escherichia coli
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The plasmid containing the ethanolamine ammonia-lyase coding sequence is extracted from the Escherichia coli overexpression strain. Site-directed mutagenesis is performed on the R160 position within the eutb sequence by using the GeneTailor site-directed mutagenesis kit. Five plasmids, including the four mutations R160A, R160K, R160E, and R160I and a wild-type control, are constructed and transformed into the competent Escherichia coli DH5R T1R strain.
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the plasmid pET-SEAL, encoding the small and large subunits of EAL is expressed in Escherichia coli
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