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4.3.1.3: histidine ammonia-lyase

This is an abbreviated version!
For detailed information about histidine ammonia-lyase, go to the full flat file.

Word Map on EC 4.3.1.3

Reaction

L-histidine
=
Urocanate
 +
NH3

Synonyms

ammonia-lyase, histidine, HAL, HAL1, Hal2, histidase, histidase, Hut, histidinase, histidinase-2, histidine alpha-deaminase, histidine ammonia lyase, histidine ammonia-lyase, histidine ammonia-lyase 2, histidine deaminase, L-HAL, L-histidase, L-histidine NH3-lyase

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.1 Ammonia-lyases
                4.3.1.3 histidine ammonia-lyase

Storage Stability

Storage Stability on EC 4.3.1.3 - histidine ammonia-lyase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 50% loss of activity after 4 months
-
-20°C, 0.05 M potassium phosphate buffer, pH 7.0, no significant loss of activity for 2 years
-
0-4°C, in a tube containing a few drops of chloroform
-
0°C, 24 h, pH 3.0 or pH 12.0, complete loss of activity
-
0°C, 24 h, pH 7.0-10.0, enzyme retains full activity
-
0°C, complete loss of activity within 1 week
-
2°C, 0.1 M potassium phosphate, pH 7.2, 0.1 mM MnCl2 saturated with chloroform, stable for 9 months without loss of activity
-
37°C, enzyme in solution: 50% loss of activity after 9.5 days, microencapsulated enzyme: 50% loss of activity after 15 days
-
4°C, enzyme in solution: 27% loss of activity after 21 days, and 60% loss of activity after 77 days, microencapsulated enzyme: 5% loss of activity after 21 days, and less than 50% loss of activity after 77 days
-
5°C, crystalline enzyme in 3.5 M ammonium sulfate, stable for 1 year
Achromobacter liquidum
-