4.3.1.27: threo-3-hydroxy-D-aspartate ammonia-lyase

This is an abbreviated version!
For detailed information about threo-3-hydroxy-D-aspartate ammonia-lyase, go to the full flat file.

D-THA DH, D-threo-3-hydroxyaspartate ammonia-lyase, D-threo-3-hydroxyaspartate dehydratase

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Results	in table
165	AA Sequence
1	Application
2	Cloned(Commentary)
2	Cofactor
2	Crystallization (Commentary)
2	Expression
1	General Stability
3	Inhibitors
5	kcat/KM [mM/s]
5	KM Value [mM]
8	Metals/Ions
5	Molecular Weight [Da]
3	Organism
16	PDB ID
2	pH Optimum
3	Protein Variants
2	Purification (Commentary)
1	Reaction
3	Reference
4	Specific Activity [micromol/min/mg]
9	Substrates and Products (Substrate)
1	Subunits
8	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
5	Turnover Number [1/s]

Specific Activity on EC 4.3.1.27 - threo-3-hydroxy-D-aspartate ammonia-lyase

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0.12

pH 8.0, 30°C, mutant enzyme H351A, substrate: threo-3-hydroxy-D-aspartate

0.18

cell extract, at 50°C, pH 8.5

21.1

pH 8.0, 30°C, wild-type enzyme, substrate: threo-3-hydroxy-D-aspartate

21.3

after 115.8fold purification, at 50°C, pH 8.5