4.3.1.17: L-serine ammonia-lyase

This is an abbreviated version!
For detailed information about L-serine ammonia-lyase, go to the full flat file.

Word Map on EC 4.3.1.17

4.3.1.17

aminotransferase

pyridoxal

deamination

glucagon

gluconeogenesis

gluconeogenic

hydroxymethyltransferase

l-isoleucine

d-serine

plp-dependent

peptostreptococcus

deaminases

2-oxobutyrate

asaccharolyticus

analysis

medicine

Reaction

Synonyms

bsLSD type 1, cSDH, Dehydratase, L-serine, EC 4.2.1.13, GSU0486, HSDH, L-Hydroxy amino acid dehydratase, L-SD, L-SD1, L-SD2, L-serine ammonia-lyase, L-Serine deaminase, L-serine deaminase 2, L-serine dehydratase, L-serine dehydratase 1, L-serine dehydratase 2, L-threonine deaminase, L-threonine dehydratase, lpLSD, lpLSD type 2, More, MSMEG_3532, PH0054, SD, SdaA, SdaB, SDH, SDH-like-1, SDH1, SDH2, SdhA, SerDH, Serine deaminase, Serine dehydratase, serine dehydratase like-1, tdcB, TdcG

ECTree

4 Lyases

4.3 Carbon-nitrogen lyases

4.3.1 Ammonia-lyases

4.3.1.17 L-serine ammonia-lyase

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Results	in table
4	Activating Compound
35908	AA Sequence
3	Application
1	CAS Registry Number
14	Cloned(Commentary)
33	Cofactor
8	Crystallization (Commentary)
89	Disease/ Diagnostics
2	General Information
5	General Stability
1	IC50 Value
72	Inhibitors
24	kcat/KM [mM/s]
7	Ki Value [mM]
83	KM Value [mM]
3	Localization
32	Metals/Ions
66	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
90	Organism
6	Oxidation Stability
8	Pathway
49	PDB ID
24	pH Optimum
5	pH Range
6	pH Stability
1	pI Value
43	Protein Variants
37	Purification (Commentary)
7	Reaction
5	Reaction Type
84	Reference
27	Source Tissue
32	Specific Activity [micromol/min/mg]
10	Storage Stability
139	Substrates and Products (Substrate)
30	Subunits
90	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
1	Temperature Range [°C]
13	Temperature Stability [°C]
42	Turnover Number [1/s]

Purification

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Purification on EC 4.3.1.17 - L-serine ammonia-lyase

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8 entries

anaerobic purification of His-tagged enzyme, purified protein is brown in colour

Escherichia coli

664943

both isoforms A and B

Mus musculus

662950

cSDH is purified using a Sephacryl S-200 and a Ni2+-NAT column

Homo sapiens

P20132

678411

expression in Escherichia coli with N-terminal His-tag, purification protocol from inclusion bodies

Homo sapiens

663323

immobilized metal ion affinity chromatography (Ni2+)

Entamoeba histolytica

partial

partially purified

proteins of crude extracts are fractionated using a DEAE-Sephadex A-25 column

4 entries

recombinant cancer cell isozyme cSDH to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange and nickel affinity chromatography

Homo sapiens

Q96GA7

691069

recombinant enzyme expressed in insect cells

Mus musculus

Q9QZX7

661725

recombinant His-tagged SdaA

Campylobacter jejuni

651494

recombinant protein expressed in Escherichia coli

2 entries

recombinant protein, purification under anaerobic conditions

Escherichia coli

665546

recombinant SDH

2 entries

strain AJ-3170

Corynebacterium glyciniphilum

210788

use of gene fusion of the structural gene sdaA to purify L-serine deaminase 1

Escherichia coli

210795

using cobalt-based affinity columns

2 entries

using cobalt-based Talon immobilized metal affinity columns

Legionella pneumophila

729219

using Ni-NTA chromatography

2 entries