4.2.99.21: isochorismate lyase

This is an abbreviated version!
For detailed information about isochorismate lyase, go to the full flat file.

Reaction

Synonyms

pyochelin biosynthetic protein PchB, IPL, Irp9, isochorismate pyruvate lyase, isochorismate-pyruvate lyase, MbtI, PchA, PchB, PRXR1, salicylate biosynthesis protein pchB, salicylate synthase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.99 Other carbon-oxygen lyases

                4.2.99.21 isochorismate lyase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
76	AA Sequence
5	Application
1	CAS Registry Number
9	Cloned(Commentary)
1	Cofactor
9	Crystallization (Commentary)
1	Expression
23	General Information
84	Inhibitors
32	kcat/KM [mM/s]
16	Ki Value [mM]
32	KM Value [mM]
8	Metals/Ions
9	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
41	Organism
3	Pathway
12	PDB ID
3	pH Optimum
3	pH Range
1	pI Value
38	Protein Variants
3	Purification (Commentary)
14	Reaction
37	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
7	Subunits
44	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
34	Turnover Number [1/s]

Application

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Application on EC 4.2.99.21 - isochorismate lyase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Application Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

agriculture

Pseudomonas aeruginosa

-

expression of a fusion of genes pchA and pchB from Pseudomonas aeruginosa, which encode isochorismate synthase and isochorismate pyruvate-lyase, in Arabidopsis thaliana, with targeting of the gene product either to the cytosol, c-SAS plants, or to the chloroplast, p-SAS plants. In p-SAS plants, the amount of free and conjugated SA is increased more than 20fold above wild type level. P-SAS plants show a strongly dwarfed phenotype and produce very few seeds. Targeting of SAS to the cytosol causes a slight increase in free salicylic acid and a significant threefold increase in conjugated salicylic acid. The modest increase in total salicylic content does not strongly induce the resistance marker PR-1, but results in enhanced disease resistance towards a virulent isolate of Peronospora parasitica. Increased resistance of c-SAS lines is paralleled with reduced seed production

710277

biotechnology

Pseudomonas aeruginosa

-

alternative computational rational approach to improve the secondary catalytic activity of enzymes, taking as a test case the IPL enzyme. The approach is based on the use of molecular dynamic simulations employing hybrid quantum mechanics/molecular mechanics methods that allow describing breaking and forming bonds

708868

drug development

2 entries

synthesis

Pseudomonas aeruginosa

-

alternative computational rational approach to improve the secondary catalytic activity of enzymes, taking as a test case the IPL enzyme. The approach is based on the use of molecular dynamic simulations employing hybrid quantum mechanics/molecular mechanics methods that allow describing breaking and forming bonds

708868

drug development

Mycobacterium tuberculosis

P9WFX1

enzyme MbtI represents an appealing target for development of inhibitors of mycobactin biosynthesis since it is structurally and biochemically characterized, has no human orthologues, and is conditionally essential under iron-deficient conditions. Inhibitors are designed against the isochorismatase activity of the enzyme (EC 5.4.4.2)

748448

drug development

Mycobacterium tuberculosis ATCC 25618

-

enzyme MbtI represents an appealing target for development of inhibitors of mycobactin biosynthesis since it is structurally and biochemically characterized, has no human orthologues, and is conditionally essential under iron-deficient conditions. Inhibitors are designed against the isochorismatase activity of the enzyme (EC 5.4.4.2)

-