4.2.99.20: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
This is an abbreviated version!
For detailed information about 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase, go to the full flat file.
Word Map on EC 4.2.99.20
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4.2.99.20
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menaquinone
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o-succinylbenzoate
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isochorismate
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mend
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garrett
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enolase
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lipid-soluble
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thiamin
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diphosphate-dependent
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decarboxylases
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exergonic
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dioxide
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thdp-dependent
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enediolate
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phylloquinone
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gerlt
- 4.2.99.20
- menaquinone
- o-succinylbenzoate
- isochorismate
-
mend
-
garrett
- enolase
-
lipid-soluble
- thiamin
-
diphosphate-dependent
- decarboxylases
-
exergonic
- dioxide
-
thdp-dependent
-
enediolate
- phylloquinone
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gerlt
Reaction
Synonyms
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase, EC 2.5.1.64, MenH, SHCHC, SHCHC synthase, YfbB
ECTree
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Reaction
Reaction on EC 4.2.99.20 - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
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5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate
5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate
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5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate
the nucleophilicity of the catalyitc serine-histidine-aspartate triad is shielded and its catalytic role is limited to being a specific general base by an open-closed conformational change. The enzyme adopts an open conformation without a functional triad in its ligand-free form and a closed conformation with a fully functional catalytic triad in the presence of its reaction product. The open-to-closed conformational transition involves movement of half of the alpha-helical cap domain, which causes extensive structural changes in the apha/beta-domain and forces the side chainof the triad histidine to adopt an energetically disfavored gauche conformation to form the functional triad. The inactive open conformation without a triad prevails in ligand-free solution and is converted to the closed conformation with a properly formed triad by the reaction product